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リコンビナントRabMAb

Anti-Ionotropic Glutamate receptor 2 抗体 [EP966Y] (ab52932)

Reviews (1)Q&A (1)References (11)
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
  • Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]
    Ionotropic Glutamate receptor 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF,IHC-Fr or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: Human brain cell lysate. Human, mouse and rat brain lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52932の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
特記事項
WB
1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
追加情報
Is unsuitable for ICC/IF,IHC-Fr or IHC-P.

ターゲット情報

  • 機能

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

  • 配列類似性

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.

  • 翻訳後修飾

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • 細胞内局在

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

画像

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution

    Lane 1 : Human brain
    Lane 2 : Rat brain
    Lane 3 : Mouse brain

    Predicted band size: 99 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (11)

ab52932 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52932 は 11 報の論文で使用されています。

  • Yao XP  et al. Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals. Brain Behav Immun 108:204-220 (2023). PubMed: 36496170
  • Xue SG  et al. Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice. Nat Commun 14:7971 (2023). PubMed: 38042894
  • Zhang SQ  et al. Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male rats. Neurobiol Stress 18:100453 (2022). PubMed: 35685681
  • Liu P & Du J Oridonin is an antidepressant molecule working through the PPAR-?/AMPA receptor signaling pathway. Biochem Pharmacol 180:114136 (2020). PubMed: 32628930
  • Huang X  et al. Methamphetamine abuse impairs motor cortical plasticity and function. Mol Psychiatry 22:1274-1281 (2017). PubMed: 28831198
View all Publications for this product

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Feb 25 2014

Question

I have a question about ab52932, Ionotropic Glutamate receptor 2 antibody [EP966Y]. What is the sequential homology between ab52932 and human GluR1 (SwissProt P42261), human GluR3 (SwissProt P42263) and human GluR4 (SwissProt P48058)? Has ab52932 tested reactivity with those human Glutamate receptors?

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Answer

Thanks for your enquiry. The sequence homology between the immunogen sequence of ab52932 and human Glutamate receptors are as follows. human GluR1: 44.4% human GluR3: 61.5% human GluR4: 56.2% Based on sequence analysis, ab52932 is most likely not reactive with other glutamate receptors (1, 3, 4). I hope this information helps you, please do not hesitate to contact us if you need any more advice or information.

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