Anti-Ionotropic Glutamate receptor 2 抗体 (ab20673)
Key features and details
- Rabbit polyclonal to Ionotropic Glutamate receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Ionotropic Glutamate receptor 2 antibody
Ionotropic Glutamate receptor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ionotropic Glutamate receptor 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Chicken, Human
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
Immunogen の所有権に関して (Peptide available as ab25708.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20673の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
|
| 特記事項 |
|---|
|
WB
Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
ターゲット情報
-
機能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
翻訳後修飾
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
-
参照データベース
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
別名
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
画像
-
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Lane 1 : Marker
Lanes 2-5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa. -
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (33)
ab20673 は 33 報の論文で使用されています。
- Iemolo A et al. Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy. NPJ Parkinsons Dis 9:92 (2023). PubMed: 37328503
- Ford L et al. CPEB3 low-complexity motif regulates local protein synthesis via protein-protein interactions in neuronal ribonucleoprotein granules. Proc Natl Acad Sci U S A 120:e2114747120 (2023). PubMed: 36716374
- Wright AL et al. The Q/R editing site of AMPA receptor GluA2 subunit acts as an epigenetic switch regulating dendritic spines, neurodegeneration and cognitive deficits in Alzheimer's disease. Mol Neurodegener 18:65 (2023). PubMed: 37759260
- Luo L et al. Caveolin-1-Mediated Cholesterol Accumulation Contributes to Exaggerated mGluR-Dependent Long-Term Depression and Impaired Cognition in Fmr1 Knockout Mice. Mol Neurobiol 60:3379-3395 (2023). PubMed: 36854997
- Niikura R et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663