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Anti-Involucrin 抗体 [SY8] (ab20202)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (ab20202)

    Key features and details

    • Mouse monoclonal [SY8] to Involucrin
    • Suitable for: IP, IHC-P, WB
    • Reacts with: Human, Pig, Gorilla
    • Isotype: IgG1

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Involucrin antibody [EPR13054] (ab181980)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Involucrin antibody [SY8]
      Involucrin 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [SY8] to Involucrin

    • 由来種

      Mouse

    • 特異性

      Keratinocytes of epidermis and other stratified squamous epithelia

    • アプリケーション

      適用あり: IP, IHC-P, WBmore details

    • 種交差性

      交差種: Human, Pig, Gorilla

    • 免疫原

      Pure involucrin from human keratinocytes.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.02% Sodium azide
      Constituent: 99.98% PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      SY8

    • ミエローマ

      P3x63

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab20202の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IP
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    特記事項
    IP
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

    • 組織特異性

      Keratinocytes of epidermis and other stratified squamous epithelia.

    • 配列類似性

      Belongs to the involucrin family.

    • 翻訳後修飾

      Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

    • 細胞内局在

      Cytoplasm. Constituent of the scaffolding of the cornified envelope.
    • Information by UniProt

    • 参照データベース

      • 別名

        • INVO_HUMAN antibody
        • Involucrin antibody
        • IVL antibody

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (ab20202)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (ab20202)
        Ab20202 staining Human skin Staining is localised to the cytoplasm.
        Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      参考文献 (2)

      ab20202 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab20202 は 2 報の論文で使用されています。

      • Goren I  et al. Increase of cystathionine-?-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes. Nitric Oxide 87:31-42 (2019). PubMed: 30862476
      • Buerger C  et al. Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation. PLoS One 12:e0180853 (2017). Human . PubMed: 28700632

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