Anti-Involucrin 抗体 [SY5] (ab68)
Key features and details
- Mouse monoclonal [SY5] to Involucrin
- Suitable for: ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Involucrin antibody [SY5]
Involucrin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SY5] to Involucrin -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cytmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Human Involucrin. Purified involucrin from human keratinocytes.
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エピトープ
The epitope maps between codon 421-568 of human involucrin. -
ポジティブ・コントロール
- IHC-P: Human tonsil, skin tissue. Localized to upper spinous and granular layers in normal skin. Flow cyt: A431 cells. ICC/IF: MCF7 cells.
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特記事項
Involucrin is the differentiation marker of human keratinocytes.
ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C (Note than enzyme digestion is better than citrate buffer based epitope unmasking)
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
SY5 -
ミエローマ
P3x63 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P | (2) |
Use a concentration of 0.1 - 0.2 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 0.1 - 0.2 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
組織特異性
Keratinocytes of epidermis and other stratified squamous epithelia. -
配列類似性
Belongs to the involucrin family. -
翻訳後修飾
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
細胞内局在
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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参照データベース
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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別名
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)This image is courtesy of an anonymous abreview.ab68 at a 1/1000 dilution staining Involucrin in human normal skin tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 2 hours at 21°C. Heat mediated antigen retrieval step performed using citrate buffer pH 6.0. Blocked with 10% serum for 30 minutes at 21°C. Secondary used at 1/100 polyclonal Donkey anti-mouse IgG conjugated to Alexa Fluor 594 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)Chavez-Munoz, C. et al PLoS One. 2013 Dec 2;8(12):e80587. doi: 10.1371/journal.pone.0080587. eCollection 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
KLC (keratinocyte-like cells) have the capacity to stratify
Human skin was decellularized and confirmed complete cell removal by H&E staining. ASC (human adipose-derived stem cells) were seeded on the epithelial side of human dermal decellularized matrices in the presence or absence of EGF (0.005 µg/mL). Human dermal decellularized matrices seeded with keratinocytes were used as controls. All three groups were kept in the same media conditions and lifted to an air-liquid interphase at the same time. Histological analysis revealed the presence of several layers of KLC with an organization similar to that of keratinocytes (A.1and 3). Surprisingly, ASC without EGF not only did not transdifferentiate into KLC, but instead migrated inside the matrix (A.2). Immunohistochemical analysis revealed a positive staining of cytokeratin-5 (B.3), cytokeratin-10 (C.3) and Involucrin (D.3) in stratified KLC matrices comparable to that of the stratified keratinocyte matrix (A, B, C and D.1). No positive staining was found in the ASC seeded matrices (A, B, C and D.2).
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Overlay histogram showing A431 cells stained with ab68 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ICC/IF image of ab68 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
Immunohistochemical analysis of Formalin fixed paraffin embedded human tonsil section labelling Involucrin with ab68 at dilution of 0.1 ug/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab68 は 49 報の論文で使用されています。
- Pappalardo A et al. Engineering edgeless human skin with enhanced biomechanical properties. Sci Adv 9:eade2514 (2023). PubMed: 36706190
- Kim HS et al. β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling. Sci Rep 13:22357 (2023). PubMed: 38102220
- Cabaço LC et al. RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium. JID Innov 2:100139 (2022). PubMed: 36090299
- Zuniga K et al. Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels. Bioengineering (Basel) 9:N/A (2022). PubMed: 36290526
- Daugelaviciene N et al. Lysosome-targeted photodynamic treatment induces primary keratinocyte differentiation. J Photochem Photobiol B 218:112183 (2021). PubMed: 33831753