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リコンビナントRabMAb

Anti-Involucrin 抗体 [EPR13060(N)] - C-terminal (ab184969)

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Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
  • Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
  • Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
  • Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13060(N)] to Involucrin - C-terminal
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Involucrin antibody [EPR13060(N)] - C-terminal
    Involucrin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13060(N)] to Involucrin - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal skin and HT-1080 cell lysates; HACAT cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13060(N)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184969の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 68 kDa).
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 68 kDa).
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

  • 組織特異性

    Keratinocytes of epidermis and other stratified squamous epithelia.

  • 配列類似性

    Belongs to the involucrin family.

  • 翻訳後修飾

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

  • 細胞内局在

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt

  • 参照データベース

    • 別名

      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    画像

    • Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/10000 dilution + Human fetal skin tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 68 kDa

    • Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)

      Intracellular flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution + HT-1080 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 68 kDa

    • Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)
      Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)

    参考文献 (0)

    ab184969 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184969 は論文での使用が確認できていません。

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