Anti-Involucrin 抗体 (ab27495)
Key features and details
- Rabbit polyclonal to Involucrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Involucrin antibody
Involucrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Involucrin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human Involucrin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P07476 -
ポジティブ・コントロール
- Human skin
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27495の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
組織特異性
Keratinocytes of epidermis and other stratified squamous epithelia. -
配列類似性
Belongs to the involucrin family. -
翻訳後修飾
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
細胞内局在
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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参照データベース
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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別名
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab27495 は 9 報の論文で使用されています。
- Pappalardo A et al. Engineering edgeless human skin with enhanced biomechanical properties. Sci Adv 9:eade2514 (2023). PubMed: 36706190
- Fontan CT et al. A Critical Role for p53 during the HPV16 Life Cycle. Microbiol Spectr 10:e0068122 (2022). PubMed: 35608342
- Prabhakar AT et al. CK2 Phosphorylation of Human Papillomavirus 16 E2 on Serine 23 Promotes Interaction with TopBP1 and Is Critical for E2 Interaction with Mitotic Chromatin and the Viral Life Cycle. mBio 12:e0116321 (2021). PubMed: 34544280
- Hieda DS et al. Air Particulate Matter Induces Skin Barrier Dysfunction and Water Transport Alteration on a Reconstructed Human Epidermis Model. J Invest Dermatol N/A:N/A (2020). PubMed: 32339540
- Camarena DEM et al. Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing. Nanomaterials (Basel) 10:N/A (2020). PubMed: 33339410
- Evans MR et al. An oral keratinocyte life cycle model identifies novel host genome regulation by human papillomavirus 16 relevant to HPV positive head and neck cancer. Oncotarget 8:81892-81909 (2017). IHC-P ; Human . PubMed: 29137231
- Melo SP et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther 22:725-33 (2014). ICC/IF ; Human . PubMed: 24390279
- Lamb R & Ambler CA Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLoS One 8:e52494 (2013). ICC/IF ; Human . PubMed: 23326335
- Brohem CA et al. Proteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death. Pigment Cell Melanoma Res 25:354-69 (2012). PubMed: 22372875