Anti-INTS11 抗体 (ab75276)
Key features and details
- Rabbit polyclonal to INTS11
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-INTS11 antibody
INTS11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to INTS11 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human INTS11.
Database link: NP_060341.2 -
ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab75276 was affinity purified using an epitope specific to INTS11 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75276の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. -
配列類似性
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily. -
ドメイン
The HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 54973 Human
- Entrez Gene: 71957 Mouse
- Entrez Gene: 100171611 Orangutan
- Omim: 611354 Human
- SwissProt: Q5TA45 Human
- SwissProt: Q9CWS4 Mouse
- SwissProt: Q5NVE6 Orangutan
- Unigene: 6449 Human
see all -
別名
- 2410006F12Rik antibody
- AU044035 antibody
- Cleavage and polyadenylation specific factor 3 like antibody
see all
画像
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Western blot - Anti-INTS11 antibody (ab75276)All lanes : Anti-INTS11 antibody (ab75276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS11 antibody (ab75276)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INTS11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-INTS11 antibody (ab75276)Detection of INTS11 by Western Blot of Immunprecipitate.
ab75278 at 1µg/ml staining INTS11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab75276 は 7 報の論文で使用されています。
- Kirstein N et al. The Integrator complex regulates microRNA abundance through RISC loading. Sci Adv 9:eadf0597 (2023). PubMed: 36763664
- Bragado L et al. SUMO conjugation regulates the activity of the Integrator complex. Nucleic Acids Res 50:12444-12461 (2022). PubMed: 36454007
- Rosa-Mercado NA et al. Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression. Mol Cell 81:502-513.e4 (2021). PubMed: 33400923
- Barra J et al. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Sci Adv 6:eaaz9072 (2020). PubMed: 32923585
- Xie M et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev 29:1552-64 (2015). PubMed: 26220997
- O'Reilly D et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24097444
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). PubMed: 24360278