AB232638

Anti-intestinal alkaline phosphatase 抗体 [EPR15201(B)] - BSA and Azide free

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PPBI antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

別名を表示する

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186422).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

This data was developed using ab186422, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling intestinal alkaline phosphatase with ab186422 at 1/100 dilution.

Positive staining on brush border of mouse small intestine.

The section was incubated with ab186422 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

This data was developed using ab186422, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling intestinal alkaline phosphatase with ab186422 at 1/100 dilution.

Negative control : no staining on mouse skeletal muscle.

The section was incubated with ab186422 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15201(B)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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製品の詳細

ab232638 is the carrier-free version of ab186422.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Intestinal alkaline phosphatase also known as alkaline phosphatase intestinal or intestinal ALP is an enzyme that plays an important mechanical role in dephosphorylation processes. This enzyme with a molecular weight (mw) of approximately 57 kDa is primarily expressed in the brush border of enterocytes lining the intestinal tract. It exhibits phosphatase activity by removing phosphate groups from various molecules including nucleotides proteins and alkaloids to regulate diverse physiological processes.

Biological function summary

Intestinal alkaline phosphatase is involved in maintaining gut health and homeostasis. It participates in the detoxification of lipopolysaccharides (LPS) and modulation of gut microbiota thereby reducing inflammation and protecting intestinal barrier function. This enzyme does not form part of a complex; instead it operates as a monomeric protein to execute its functions. By dephosphorylating toxins it contributes to the defense mechanisms of the intestinal mucosa aiding in the preservation of a balanced intestinal environment.

Pathways

Intestinal alkaline phosphatase is integrated into the digestive and immune pathways. Its function is closely linked with the regulation of the Toll-like receptor signaling pathway by detoxifying LPS a major outer membrane component of Gram-negative bacteria. Through this pathway it associates with proteins such as TLR4 thereby influencing immune responses and cytokine production. Additionally it plays a role in the absorption of nutrients by interacting with other digestive enzymes contributing to efficient nutrient uptake and utilization.

Intestinal alkaline phosphatase has been implicated in conditions such as inflammatory bowel disease (IBD) and metabolic syndrome. In inflammatory bowel disease the enzyme's ability to dephosphorylate and detoxify LPS is significant in ameliorating intestinal inflammation. Moreover an association exists between inadequate intestinal alkaline phosphatase activity and metabolic syndrome linking it to proteins such as LPS-binding protein (LBP) involved in inflammation and metabolism. Consequently this enzyme emerges as a potential therapeutic target in managing these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPI

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9664 PubMed39511206

2024

mRNA delivery enabled by metal-organic nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuang Gu,Jingqu Chen,Zhaoran Wang,Chang Liu,Tianzheng Wang,Chan-Jin Kim,Helena Durikova,Soraia Fernandes,Darryl N Johnson,Robert De Rose,Christina Cortez-Jugo,Frank Caruso

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-intestinal alkaline phosphatase 抗体 [EPR15201(B)] - BSA and Azide free

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise