Anti-intestinal alkaline phosphatase 抗体 [EPR15201(B)] (ab186422)
![Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)](https://www.abcam.co.jp/ps/products/186/ab186422/Images/ab186422-297166-anti-intestinal-alkaline-phosphatase-antibody-epr15201b-western-blot.jpg "Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]
intestinal alkaline phosphatase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15201(B)] to intestinal alkaline phosphatase -
由来種
Rabbit -
特異性
The immunogen used for this product shares 85.7% homology with ALPPL2 (Alkaline phosphatase placental-like). Cross-reactivity with this protein has not been confirmed experimentally. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, JAR and Human placenta lysate; Human small intestine tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15201(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 57 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the alkaline phosphatase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 248 Human
- Omim: 171740 Human
- SwissProt: P09923 Human
- Unigene: 284255 Human
- Unigene: 37009 Human
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別名
- Alkaline phosphatase intestinal antibody
- Alkaline phosphomonoesterase antibody
- Alpi antibody
see all
画像
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Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human placenta lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 15 secondsBlocking and Diluting buffer: 5% NFDM/TBST
This antibody does not work in Caco-2 cells which should be positive (PMID: 20660763).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)](https://www.abcam.co.jp/ps/products/186/ab186422/Images/ab186422-220017-anti-intestinal-alkaline-phosphatase-antibody-epr15201b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)](https://www.abcam.co.jp/ps/products/186/ab186422/Images/ab186422-220019-anti-intestinal-alkaline-phosphatase-antibody-epr15201b-western-blot.jpg)
Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa -
![Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)](https://www.abcam.co.jp/ps/products/186/ab186422/Images/ab186422-220018-anti-intestinal-alkaline-phosphatase-antibody-epr15201b-western-blot.jpg)
Western blot - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)All lanes : Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa -
![Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)](https://www.abcam.co.jp/ps/products/186/ab186422/Images/ab186422-5-benefits-of-recombinant-antibodies.png)
Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (ab186422)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab186422 は 7 報の論文で使用されています。
- Thibault MP et al. Lipocalin-2 and calprotectin as stool biomarkers for predicting necrotizing enterocolitis in premature neonates. Pediatr Res 91:129-136 (2022). PubMed: 34465872
- Takeuchi A et al. IIAEK Targets Intestinal Alkaline Phosphatase (IAP) to Improve Cholesterol Metabolism with a Specific Activation of IAP and Downregulation of ABCA1. Nutrients 12:N/A (2020). PubMed: 32961978
- Good C et al. Type III interferon signaling restricts enterovirus 71 infection of goblet cells. Sci Adv 5:eaau4255 (2019). PubMed: 30854425
- Bing T et al. Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers. Adv Sci (Weinh) 6:1900143 (2019). PubMed: 31179220
- Zhou D et al. Valproic acid prevents glucocorticoid-induced osteonecrosis of the femoral head of rats. Int J Mol Med 41:3433-3447 (2018). PubMed: 29512684
- Ning S et al. Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress. Int J Mol Med 39:135-143 (2017). PubMed: 28004120
- Cui Y et al. Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells. Cell Physiol Biochem 39:1347-59 (2016). PubMed: 27607236