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AB97532

Anti-intestinal alkaline phosphatase 抗体

Anti-intestinal alkaline phosphatase antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal PPBI antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALPI aa 1-250.

別名を表示する

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody (AB97532)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody (AB97532)

Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab97532 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody (AB97532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody (AB97532)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue stained for Alkaline phosphatase (intestinal) protein at cell membrane with ab97532 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human ALPI aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P09923

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代替製品

Primary Antibodies

AB186422

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] (AB186422)

  • 1
  • 1
Primary Antibodies

AB232638

Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody [EPR15201(B)] - BSA and Azide free (AB232638)

  • 0
  • 0
Primary Antibodies

AB118816

Anti-intestinal alkaline phosphatase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-intestinal alkaline phosphatase antibody (AB118816)

  • 0
  • 0
Primary Antibodies

AB18111

Anti-intestinal alkaline phosphatase antibody [AAP.1]

  • 0
  • 0

組合せ製品

Primary Antibodies

AB305305

Anti-Alkaline Phosphatase antibody [EPR27506-72]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry - Anti-Alkaline Phosphatase antibody [EPR27506-72] (AB305305)

  • 0
  • 0
Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

  • 5
  • 7
Primary Antibodies

AB75850

Anti-LGR5 antibody [EPR3065Y]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

  • 4
  • 7
Primary Antibodies

AB216327

Anti-Occludin antibody [EPR20992]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (AB216327)

  • 4
  • 7
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Intestinal alkaline phosphatase also known as alkaline phosphatase intestinal or intestinal ALP is an enzyme that plays an important mechanical role in dephosphorylation processes. This enzyme with a molecular weight (mw) of approximately 57 kDa is primarily expressed in the brush border of enterocytes lining the intestinal tract. It exhibits phosphatase activity by removing phosphate groups from various molecules including nucleotides proteins and alkaloids to regulate diverse physiological processes.
Biological function summary

Intestinal alkaline phosphatase is involved in maintaining gut health and homeostasis. It participates in the detoxification of lipopolysaccharides (LPS) and modulation of gut microbiota thereby reducing inflammation and protecting intestinal barrier function. This enzyme does not form part of a complex; instead it operates as a monomeric protein to execute its functions. By dephosphorylating toxins it contributes to the defense mechanisms of the intestinal mucosa aiding in the preservation of a balanced intestinal environment.

Pathways

Intestinal alkaline phosphatase is integrated into the digestive and immune pathways. Its function is closely linked with the regulation of the Toll-like receptor signaling pathway by detoxifying LPS a major outer membrane component of Gram-negative bacteria. Through this pathway it associates with proteins such as TLR4 thereby influencing immune responses and cytokine production. Additionally it plays a role in the absorption of nutrients by interacting with other digestive enzymes contributing to efficient nutrient uptake and utilization.

Intestinal alkaline phosphatase has been implicated in conditions such as inflammatory bowel disease (IBD) and metabolic syndrome. In inflammatory bowel disease the enzyme's ability to dephosphorylate and detoxify LPS is significant in ameliorating intestinal inflammation. Moreover an association exists between inadequate intestinal alkaline phosphatase activity and metabolic syndrome linking it to proteins such as LPS-binding protein (LBP) involved in inflammation and metabolism. Consequently this enzyme emerges as a potential therapeutic target in managing these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information ALPI
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928261

2024

Intestinal Ketogenesis and Permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Casselbrant,Erik Elias,Peter Hallersund,Erik Elebring,Jakob Cervin,Lars Fändriks,Ville Wallenius

The Journal of biological chemistry 289:25306-16 PubMed25037223

2014

The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner.

Applications

WB

Species

Human

Joongho Shin,Azadeh Carr,Georgia A Corner,Lars Tögel,Mercedes Dávalos-Salas,Mercedes Dávaos-Salas,Hoanh Tran,Anderly C Chueh,Sheren Al-Obaidi,Fiona Chionh,Naseem Ahmed,Daniel D Buchanan,Joanne P Young,Madhu S Malo,Richard A Hodin,Diego Arango,Oliver M Sieber,Leonard H Augenlicht,Amardeep S Dhillon,Thomas K Weber,John M Mariadason
View all publications
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