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Anti-Interferon gamma 抗体 (ab25101)

Reviews (4)Q&A (4)References (23)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody (ab25101)
  • Sandwich ELISA - Anti-Interferon gamma antibody (ab25101)

Key features and details

  • Rabbit polyclonal to Interferon gamma
  • Suitable for: Sandwich ELISA, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Interferon gamma antibody [EPR21704] (ab231036)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Interferon gamma antibody
    Interferon gamma 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Interferon gamma

  • 由来種

    Rabbit

  • 特異性

    This antibody neutralizes both natural and recombinant human IFN gamma. Activity =10^4 Neutralizing U/mg protein.

  • アプリケーション

    適用あり: Sandwich ELISA, IHC-Pmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant full length protein corresponding to Human Interferon gamma.
    Database link: P01579

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab25101の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MD-1] to Interferon gamma (ab25014).

For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab25014 as capture.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MD-1] to Interferon gamma (ab25014).

For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab25014 as capture.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 組織特異性

    Released primarily from activated T lymphocytes.

  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • 配列類似性

    Belongs to the type II (or gamma) interferon family.

  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • ifn g antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody (ab25101)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody (ab25101)

      ab25101 staining Human tonsil. Staining is localised to the cytoplasm and protein is secreted.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be

    • Sandwich ELISA - Anti-Interferon gamma antibody (ab25101)
      Sandwich ELISA - Anti-Interferon gamma antibody (ab25101)

      Standard curve for Interferon gamma (analyte: ab51240); dilution range 1pg/ml to 1µg/ml using capture antibody ab25014 at 5µg/ml and detector antibody ab25101 at 0.5µg/ml.

    参考文献 (23)

    ab25101 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab25101 は 23 報の論文で使用されています。

    • Tittarelli A  et al. Co-Expression of Immunohistochemical Markers MRP2, CXCR4, and PD-L1 in Gallbladder Tumors Is Associated with Prolonged Patient Survival. Cancers (Basel) 15:N/A (2023). PubMed: 37444550
    • Yin J  et al. Neoadjuvant adebrelimab in locally advanced resectable esophageal squamous cell carcinoma: a phase 1b trial. Nat Med 29:2068-2078 (2023). PubMed: 37488287
    • Chen JS  et al. IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies. JID Innov 3:100189 (2023). PubMed: 37205304
    • Xia P  et al. NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis. BMC Immunol 23:39 (2022). PubMed: 35965334
    • Ma L  et al. Inflammation Mediates the Development of Aggressive Breast Cancer Following Radiotherapy. Clin Cancer Res 27:1778-1791 (2021). PubMed: 33402361
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon gamma antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (B16 Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    B16 Pancreas
    Blocking step
    Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 31 2021

    Western blot abreview for Anti-Interferon gamma antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Gel, PVDF membrane)
    Loading amount
    40 µg
    Specification
    B16 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 17 2019

    Western blot abreview for Anti-Interferon gamma antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Panc1)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Gel, PVDF membrane)
    Loading amount
    40 µg
    Specification
    Panc1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 17 2019

    Immunohistochemistry (Resin sections) abreview for Anti-Interferon gamma antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Ali Hosseini

    Verified customer

    投稿 Oct 27 2014

    Question

    Hell


    As you know we have ordered a number of anti-human ab from Abcam. The list
    is attached...
    I would like to request fro your assistance in the following...
    We would like to get the information about the recommended diluent for the
    ab to be used for IHC with paraffin embedded tissue sections since its known
    to affect results at times.
    Other information regarding antigen retrieval conditions etc will be
    available on the net, but could be refurbished if they are updated.
    Equally important is the source of positive and negative controls.
    I would especially like to request for slides of both the types of controls
    as they are vital to decide/judge the variations in the protocols. I hope
    this is done as it will serve us for a long time in future.

    Read More
    Answer

    Thank you for contacting us.

    I have attached the filled out Exel spreadsheet with all the requested information. To be fair, all the information asked could be answered with a look on the datasheet...

    In addition, I had a look through our tissue slices for her IHC-P experiments. Instead of the Tonsils she can use Lymphknot (as we do not have Tonsil slides).

    https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). PC for ab25101and ab10563

    https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). PC for ab34843

    https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). PC for ab37158

    Read More

    Question

    Thank you very much for your informative answer. A polyclonal antibody would unfortunately not be very useful for us. I wonder whether you have a pair of monoclonal antibodies that work in sandwich ELISA against human IFNy?

    That would be great! Looking forward to your answer,

    Read More
    Answer

    We have a pair of anti-IFN gamma monoclonals that have been tested in sandwich ELISA:

    Capture - ab8318

    Detection - ab10076

    Click here (or use the following: https://www.abcam.com/Interferon-gamma-antibody-H3-1-ab8318.html).

    Click here (or use the following: https://www.abcam.com/Interferon-gamma-antibody-GD4-HRP-ab10076.html).

    I hope this helps. Please let me know if you have any questions.

    Read More

    Question

    I was looking for a monoclonal antibody against human IFN y, to be used in sandwich ELISA. We already have an antibody against this target, and having a second antibody would of course enable us to potentially set up a sandwich ELISA. I noticed several antibodies on your website that could be interesting, but unfortunately, the epitope targeted is not stated. Do you maybe have an antibody with our desired specifications and a known epitope?

    Read More
    Answer

    Thank you for your interest in our IFN gamma antibodies.

    I recommend using your monoclonal antibody for capture in a sandwich ELISA, and a rabbit anti-human IFN gamma polyclonal antibody raised against full-length protein for detection, followed by an anti-rabbit IgG secondary antibody, pre-adsorbed against mouse IgG to remove reactivity with mouse (assuming your monoclonal antibody is mouse IgG). The secondary will be conjugated to HRP or AP or fluorochrome, depending on how you anticipate detecting the signal.

    I suggest ab25101 for the polyclonal IFN gamma antibody,

    Click here (or use the following: https://www.abcam.com/Interferon-gamma-antibody-ab25101.html)

    and I suggest ab97080 for the anti-rabbit secondary, assuming you use HRP plus substrate for detection.

    Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97080.html).

    The epitopes the antibody recognizes are not mapped, since this is a polyclonal antibody which recognizes multiple epitopes along the entire length of IFN gamma. If you want a monoclonal antibody (which recognizes a single epitope, rather than many) to pair with your monoclonal, I will need to know the epitope that your antibody recognizes in order to select an appropriate match. One of the two mouse monoclonal antibodies will need to be directly conjugated to an enzyme or fluorochrome, to avoid using an anti-mouse IgG secondary antibody.

    I hope this is helpful. Please let me know if you have questions.

    Read More

    Question

    Dear Sir/Madam, Our customer would like to inquire whether ab25014 and ab25101 have the larger customerized package, for example, 500ug or 1mg. Would you please help us provide this information? Thanks and best regards,

    Read More
    Answer

    Thank you for contacting us. We can certainly provide these in 500ug or 1mg size as a special request. Please let me know if you would like to place an order. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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