Anti-Interferon alpha/beta receptor 1 抗体 - BSA and Azide free (ab10739)
Key features and details
- Goat polyclonal to Interferon alpha/beta receptor 1 - BSA and Azide free
- Suitable for: Flow Cyt, ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Interferon alpha/beta receptor 1 antibody - BSA and Azide free
Interferon alpha/beta receptor 1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Interferon alpha/beta receptor 1 - BSA and Azide free -
由来種
Goat -
特異性
Based on ELISA and immunoblotting, this antibody shows no cross-reactivity with recombinant human IFNGRI and IFNGRII. -
アプリケーション
適用あり: Flow Cyt, ELISA, Neutralising, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Interferon alpha/beta receptor 1.
Database link: P17181 -
特記事項
Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method. This antibody was used as a detection antibody in sELISA and was paired with ab91466 as capture antibody. Abreview 24657.
IFNAR1 is a member of the cytokine receptor superfamily which also includes receptors for interleukins, IFN gamma, ciliary neurotrophic factor, somatotrophin, erythropoietin, nerve growth factor, tumor necrosis factor, leukemia inhibitory factor, and oncostatin M. Some members of the family have an alpha chain with either low or high ligand binding affinity and at least one beta chain involved in signal transduction with either relatively low or no ligand binding affinity. Type I interferons, alpha and beta, induce a variety of effects on target cells including antiviral, antiproliferative, and immunomodulatory activities. The alpha and beta interferons compete to bind to a common cell surface receptor, while IFN gamma binds to a distinct receptor. IFNAR1 is very responsive to type I interferons and bind to IFN beta and IFN alpha subtypes. It is also functionally involved in signal transduction because of its association with the cytoplasmic tyrosine kinase JAK1. The type I interferons, alpha and beta, are produced by leukocytes (alpha subunits), fibroblasts (beta subtypes), lymphocytes (omega subtypes), and ruminant embryos (tau subtypes). Interferon receptors are generally found on most human cell types whatever their origin, even on cells poorly responsive to interferon. IFNAR1 is expressed on the cell surface in a variety of human cell lines.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity isolated antigen specific antibody is obtained from goat anti-Interferon alpha/beta receptor 1 antiserum by immuno-specific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the peptide. -
一次抗体 備考
IFNAR1 is a member of the cytokine receptor superfamily which also includes receptors for interleukins, IFN gamma, ciliary neurotrophic factor, somatotrophin, erythropoietin, nerve growth factor, tumor necrosis factor, leukemia inhibitory factor, and oncostatin M. Some members of the family have an alpha chain with either low or high ligand binding affinity and at least one beta chain involved in signal transduction with either relatively low or no ligand binding affinity. Type I interferons, alpha and beta, induce a variety of effects on target cells including antiviral, antiproliferative, and immunomodulatory activities. The alpha and beta interferons compete to bind to a common cell surface receptor, while IFN gamma binds to a distinct receptor. IFNAR1 is very responsive to type I interferons and bind to IFN beta and IFN alpha subtypes. It is also functionally involved in signal transduction because of its association with the cytoplasmic tyrosine kinase JAK1. The type I interferons, alpha and beta, are produced by leukocytes (alpha subunits), fibroblasts (beta subtypes), lymphocytes (omega subtypes), and ruminant embryos (tau subtypes). Interferon receptors are generally found on most human cell types whatever their origin, even on cells poorly responsive to interferon. IFNAR1 is expressed on the cell surface in a variety of human cell lines. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10739の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
|
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ELISA |
Use at an assay dependent concentration.
The detection limit for recombinant human IFNAR1 is approximately 0.3 ng/well. |
|
Neutralising |
Use at an assay dependent concentration.
This will block 50 % of the binding of recombinant human IFN beta (20 ng/ml) to immobilized recombinant human IFNAR1 (1 ug/ml of a 100 ul solution coated in each well) in a functional ELISA. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
The detection limit for recombinant human IFN-u/B R1 is ~25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. The detection limit for recombinant human IFNAR1 is approximately 0.3 ng/well. |
Neutralising
Use at an assay dependent concentration. This will block 50 % of the binding of recombinant human IFN beta (20 ng/ml) to immobilized recombinant human IFNAR1 (1 ug/ml of a 100 ul solution coated in each well) in a functional ELISA. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa. The detection limit for recombinant human IFN-u/B R1 is ~25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. |
ターゲット情報
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機能
Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. -
組織特異性
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. -
配列類似性
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
翻訳後修飾
Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3454 Human
- Omim: 107450 Human
- SwissProt: P17181 Human
- Unigene: 529400 Human
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別名
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (8)
ab10739 は 8 報の論文で使用されています。
- Watson SF et al. ILF3 contributes to the establishment of the antiviral type I interferon program. Nucleic Acids Res 48:116-129 (2020). PubMed: 31701124
- Harris BD et al. Human interferon-? and interferon-? exhibit low potency and low affinity for cell-surface IFNAR and the poxvirus antagonist B18R. J Biol Chem 293:16057-16068 (2018). PubMed: 30171073
- Ugolini M et al. Recognition of microbial viability via TLR8 drives TFH cell differentiation and vaccine responses. Nat Immunol 19:386-396 (2018). PubMed: 29556002
- Yu J et al. Interferon-inducible LY6E Protein Promotes HIV-1 Infection. J Biol Chem 292:4674-4685 (2017). PubMed: 28130445
- Matheis F et al. A Bifunctional Approach of Immunostimulation and uPAR Inhibition Shows Potent Antitumor Activity in Melanoma. J Invest Dermatol 136:2475-2484 (2016). PubMed: 27498344
- Szabo A et al. TLR ligands upregulate RIG-I expression in human plasmacytoid dendritic cells in a type I IFN-independent manner. Immunol Cell Biol N/A:N/A (2014). PubMed: 24839978
- Zhong RZ et al. Effects of tannic acid on Haemonchus contortus larvae viability and immune responses of sheep white blood cells in vitro. Parasite Immunol 36:100-6 (2014). PubMed: 24558656
- Li R et al. IFN{gamma} regulates retinal pigment epithelial fluid transport. Am J Physiol Cell Physiol 297:C1452-65 (2009). PubMed: 19794146