Anti-Interferon alpha 1 抗体 [F18] (ab7373)
Key features and details
- Rat monoclonal [F18] to Interferon alpha 1
- Suitable for: Neutralising, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-Interferon alpha 1 antibody [F18]
Interferon alpha 1 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [F18] to Interferon alpha 1 -
由来種
Rat -
特異性
This monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon 1. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma. -
アプリケーション
適用あり: Neutralising, Flow Cyt, Inhibition Assaymore details -
種交差性
交差種: Mouse
非交差種: Human -
ポジティブ・コントロール
- Plasmacytoid dendritic cells.
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特記事項
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
0.2 µm filtered antibody solution. -
一次抗体 備考
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies. -
ポリ/モノ
モノクローナル -
クローン名
F18 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Neutralising |
Use at an assay dependent concentration.
|
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 21172865
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Inhibition Assay |
Use at an assay dependent concentration.
Useful for inhiiton in biological assays and for affinity pruification. |
|
AP |
Use at an assay dependent concentration.
|
特記事項 |
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Neutralising
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21172865 ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Inhibition Assay
Use at an assay dependent concentration. Useful for inhiiton in biological assays and for affinity pruification. |
AP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
配列類似性
Belongs to the alpha/beta interferon family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 15962 Mouse
- Entrez Gene: 230396 Mouse
- SwissProt: P01572 Mouse
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別名
- IFL antibody
- IFN alpha antibody
- IFN alpha 1/13 antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab7373 は 7 報の論文で使用されています。
- Tian J et al. 5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING. EMBO J 40:e106065 (2021). PubMed: 33615517
- Büssow K et al. ER intrabody-mediated inhibition of interferon a secretion by mouse macrophages and dendritic cells. PLoS One 14:e0215062 (2019). PubMed: 30990863
- Bi E et al. E-cadherin expression on multiple myeloma cells activates tumor-promoting properties in plasmacytoid DCs. J Clin Invest 128:4821-4831 (2018). PubMed: 30277474
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442
- Castellaneta A et al. Plasmacytoid dendritic cell-derived IFN-a promotes murine liver ischemia/reperfusion injury by induction of hepatocyte IRF-1. Hepatology 60:267-77 (2014). PubMed: 24493010
- Björck P et al. Plasmacytoid dendritic cell dichotomy: identification of IFN-a producing cells as a phenotypically and functionally distinct subset. J Immunol 186:1477-85 (2011). Flow Cyt ; Mouse . PubMed: 21172865
- Joshi VD et al. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J Interferon Cytokine Res 26:739-47 (2006). PubMed: 17032168