Anti-Integrin beta 5 抗体 [EPR19170] (ab184312)
![Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.co.jp/ps/products/184/ab184312/Images/ab184312-276168-anti-integrin-beta-5-antibody-epr19170-western-blot.jpg "Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19170] to Integrin beta 5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Integrin beta 5 antibody [EPR19170]
Integrin beta 5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19170] to Integrin beta 5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19170 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184312の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
ターゲット情報
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機能
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3693 Human
- Entrez Gene: 16419 Mouse
- Entrez Gene: 257645 Rat
- Omim: 147561 Human
- SwissProt: P18084 Human
- SwissProt: O70309 Mouse
- Unigene: 536663 Human
- Unigene: 6424 Mouse
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別名
- FLJ26658 antibody
- Integrin beta-5 antibody
- Integrin subunit beta 5 antibody
see all
画像
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Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4 : Mouse lung lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.
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![Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.co.jp/ps/products/184/ab184312/Images/ab184312-276167-anti-integrin-beta-5-antibody-epr19170-western-blot.jpg)
Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute
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![Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.co.jp/ps/products/184/ab184312/Images/ab184312-3-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab184312 は 7 報の論文で使用されています。
- Qiu X et al. Lactobacillus rhamnosus GG alleviates colitis caused by chemotherapy via biofilm formation. J Gastroenterol Hepatol 38:1158-1169 (2023). PubMed: 36878870
- Wen X et al. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. Front Oncol 12:887068 (2022). PubMed: 36249018
- Wang X et al. Succinylation Inhibits the Enzymatic Hydrolysis of the Extracellular Matrix Protein Fibrillin 1 and Promotes Gastric Cancer Progression. Adv Sci (Weinh) 9:e2200546 (2022). PubMed: 35901491
- Tang C et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol 4:161 (2021). PubMed: 33547385
- Huang C et al. RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin ß5 axis. Life Sci 267:118928 (2021). PubMed: 33358902
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
- Chen J et al. Remarkable Boron Delivery Of iRGD-Modified Polymeric Nanoparticles For Boron Neutron Capture Therapy. Int J Nanomedicine 14:8161-8177 (2019). PubMed: 31632025