Anti-Integrin beta 4 抗体 [M126] (ab29042)
Key features and details
- Mouse monoclonal [M126] to Integrin beta 4
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Integrin beta 4 antibody [M126]
Integrin beta 4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M126] to Integrin beta 4 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.
-
ポジティブ・コントロール
- Flow Cyt (intra):A431 cell lysate. WB: 804G cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
精製度
Protein A purified -
特記事項(精製)
protein A chromatography. -
ポリ/モノ
モノクローナル -
クローン名
M126 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab29042の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
|
Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
WB
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
組織特異性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
関連疾患
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
ドメイン
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
-
別名
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
画像
-
Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min
Predicted band size: 202 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (27)
ab29042 は 27 報の論文で使用されています。
- Kechagia Z et al. The laminin-keratin link shields the nucleus from mechanical deformation and signalling. Nat Mater 22:1409-1420 (2023). PubMed: 37709930
- Mezawa M et al. TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells. Cell Adh Migr 16:13-24 (2022). PubMed: 35137648
- Tan ML et al. Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair. Int J Biol Sci 18:1254-1270 (2022). PubMed: 35173551
- Jiang LT et al. Aberrant Neuregulin 1/ErbB Signaling in Charcot-Marie-Tooth Type 4D Disease. Mol Cell Biol 42:e0055921 (2022). PubMed: 35708320
- Romo-Valera C et al. Characterisation of corneas following different time and storage methods for their use as a source of stem-like limbal epithelial cells. Exp Eye Res 211:108720 (2021). PubMed: 34389315