Anti-Integrin beta 3 (phospho Y773) 抗体 (ab38460)
Key features and details
- Rabbit polyclonal to Integrin beta 3 (phospho Y773)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Integrin beta 3 (phospho Y773) antibody
Integrin beta 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Integrin beta 3 (phospho Y773) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Integrin beta 3 (phospho Y773).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 87 kDa.
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 87 kDa. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
組織特異性
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. -
関連疾患
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3690 Human
- Omim: 173470 Human
- SwissProt: P05106 Human
- Unigene: 218040 Human
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別名
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
see all
画像
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Western blot - Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)All lanes : Anti-Integrin beta 3 (phospho Y773) antibody (ab38460) at 1/500 dilution
Lane 1 : Untreated extracts from HL-60 cells
Lane 2 : Hydrogen peroxide treated extracts from HL-60 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
Observed band size: >118 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)Immunohistochemical analysis of Integrin beta 3 (phospho Y773) expression in paraffin embedded human breast carcinoma tissue sections using 1/50 ab38460. Left: untreated sample. Right: Sample pretreated with phosphopeptide (negative control).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab38460 は 9 報の論文で使用されています。
- Lickert S et al. Platelets drive fibronectin fibrillogenesis using integrin αIIbβ3. Sci Adv 8:eabj8331 (2022). PubMed: 35275711
- Dai B et al. Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis. Nat Commun 13:427 (2022). PubMed: 35058428
- Danilucci TM et al. Recombinant RGD-disintegrin DisBa-01 blocks integrin avß3 and impairs VEGF signaling in endothelial cells. Cell Commun Signal 17:27 (2019). PubMed: 30894182
- Cammalleri M et al. The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model. J Cell Mol Med 23:5176-5192 (2019). PubMed: 31251468
- Guo S et al. GnRH agonist improves pregnancy outcome in mice with induced adenomyosis by restoring endometrial receptivity. Drug Des Devel Ther 12:1621-1631 (2018). PubMed: 29922037
- Jia D et al. Osteoprotegerin Disruption Attenuates HySu-Induced Pulmonary Hypertension Through Integrin avß3/FAK/AKT Pathway Suppression. Circ Cardiovasc Genet 10:N/A (2017). PubMed: 28077433
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467
- Sun Y et al. Carbon disulfide exposure at peri-implantation disrupts embryo implantation by decreasing integrin ß3 expression in the uterine tissue of pregnant mice. Chem Biol Interact 206:126-133 (2013). PubMed: 24025711
- Naik MU et al. JAM-A protects from thrombosis by suppressing integrin aIIbß3-dependent outside-in signaling in platelets. Blood 119:3352-60 (2012). WB ; Mouse . PubMed: 22271446