Anti-Integrin beta 3 抗体 [Y2/51] (ab9509)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Y2/51] to Integrin beta 3
- Suitable for: Flow Cyt
- Reacts with: Human
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Integrin beta 3 antibody [Y2/51]
Integrin beta 3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Y2/51] to Integrin beta 3 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC/IF,IHC-P or WB -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin beta 3. PHA-stimulated human mononuclear cells.
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ポジティブ・コントロール
- Flow cyto: Human peripheral blood mononuclear cell (PBMC) cells
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特記事項
This product has been switch from hybridoma to recombinant on 25th March 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y2/51 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
1/1000.
|
特記事項 |
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Flow Cyt
1/1000. |
ターゲット情報
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機能
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
組織特異性
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. -
関連疾患
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3690 Human
- Omim: 173470 Human
- SwissProt: P05106 Human
- Unigene: 218040 Human
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別名
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
see all
画像
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.
Goat anti mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab9509. Then stained with anti-CD41 conjugated to APC.
Gated on viable cells. -
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG / Black isotype control and an unlabelled control (Cell without incubation with primary antibody and secondary antibody / Blue).
Secondary antibody was Goat anti mouse IgG (Alexa Fluor® 488, ab150113) used at 1/2000 dilution.
Negative control: HeLa.
Gated on viable cells.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab9509 は論文での使用が確認できていません。