Anti-Integrin alpha V+beta 5 抗体 [P1F6] (ab177004)
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)](https://www.abcam.co.jp/ps/products/177/ab177004/Images/ab177004-5-anti-integrin-alpha-v-beta-5-antibody-immunocytochemistry-a549-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)")
Key features and details
- Mouse monoclonal [P1F6] to Integrin alpha V+beta 5
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Integrin alpha V+beta 5 antibody [P1F6]
Integrin alpha V+beta 5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [P1F6] to Integrin alpha V+beta 5 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin alpha V+beta 5. Small cell lung carcinoma cells (UCLA P3).
-
ポジティブ･コントロール
- This antibody gave a positive signal in IF and Flow Cytometry in A549 cells.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
P1F6 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177004の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | (1) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| ICC/IF | (2) |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
関連性
This protein belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 3685 Human
- Entrez Gene: 3693 Human
- Omim: 147561 Human
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P18084 Human
-
別名
- CD51 antibody
- Integrin alpha V antibody
- Integrin beta 5 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)
ab177004 staining Integrin alpha V+beta 5 in A549 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab177004 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
![Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)](https://www.abcam.co.jp/ps/products/177/ab177004/Images/ab177004-392818-ab177004FLOW2.jpg)
Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)Flow Cytometry Overlay histogram showing A549 cells stained with ab177004 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab177004) (1x106 cells in 100µl at 1µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.
Isotype control antibody (black line) was mouse IgG1κ; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
-
![Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)](https://www.abcam.co.jp/ps/products/177/ab177004/Images/ab177004-204811-anti-integrin-alpha-vbeta-5-antibody-p1f6-flow-cytometry.jpg)
Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)Overlay histogram showing A549 cells stained with ab177004 (red line). The cells were fixed with 4% formaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab177004, 0.1μg/1x106 cells) for 30 min at 22ºC.
The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (12)
ab177004 は 12 報の論文で使用されています。
- Grunwald V et al. Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37833934
- Ruan Z et al. Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cells. J Neuroinflammation 20:285 (2023). PubMed: 38037097
- Hashiguchi S et al. CCN2-induced lymphangiogenesis is mediated by the integrin αvβ5-ERK pathway and regulated by DUSP6. Sci Rep 12:926 (2022). PubMed: 35042954
- Lee H et al. CD82 attenuates TGF-β1-mediated epithelial-mesenchymal transition by blocking smad-dependent signaling in ARPE-19 cells. Front Pharmacol 13:991056 (2022). PubMed: 36386228
- Koehler M et al. Reovirus directly engages integrin to recruit clathrin for entry into host cells. Nat Commun 12:2149 (2021). PubMed: 33846319