Anti-Integrin alpha V 抗体 [272-17E6] - BSA and Azide free (ab16821)
Key features and details
- Mouse monoclonal [272-17E6] to Integrin alpha V - BSA and Azide free
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free
Integrin alpha V 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [272-17E6] to Integrin alpha V - BSA and Azide free -
由来種
Mouse -
特異性
Cross reacts with all alpha V integrins. -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Full length native protein (purified) corresponding to Human Integrin alpha V. Human Placental ?vß3
Database link: P06756 -
ポジティブ・コントロール
- Flow Cyt (intra): Human melanoma cells and HeLa cells
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
272-17E6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16821の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3685 Human
- Omim: 193210 Human
- SwissProt: P06756 Human
- Unigene: 436873 Human
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別名
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
画像
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Intracellular Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 µg (red), or with 1 µg of Mouse IgG isotype control.
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ab16821 staining Integrin alpha V in Human melanoma cells by intacellular Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab16821 は 29 報の論文で使用されています。
- Ghochani Y et al. A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration. Cell Rep 41:111511 (2022). PubMed: 36261010
- Assunção M et al. Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis. J Mater Chem B 9:7205-7215 (2021). PubMed: 33710248
- Steel RWJ et al. Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP. Sci Rep 11:11328 (2021). PubMed: 34059712
- Simón-Gracia L et al. Homing Peptide-Based Targeting of Tenascin-C and Fibronectin in Endometriosis. Nanomaterials (Basel) 11:N/A (2021). PubMed: 34947606
- Castro-Córdova P et al. Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Nat Commun 12:1140 (2021). PubMed: 33602902