JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB16821

Anti-Integrin alpha V 抗体 [272-17E6] - BSA and Azide free

Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free

5

(4 Reviews)

|

(29 Publications)

Mouse Monoclonal Integrin alpha V antibody. Carrier free. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGAV.

別名を表示する

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

2 Images
Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)
  • Flow Cyt (Intra)

AbReview16056****

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

ab16821 staining Integrin alpha V in Human melanoma cells by intacellular Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

This image was generated using the ascites version of the product.

This image is a courtesy of Anonymous Abreview

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (AB16821)

Intracellular Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 μg (red), or with 1 μg of Mouse IgG isotype control.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

272-17E6

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

Native Full Length Protein corresponding to Human ITGAV.

P06756

特異性

Cross reacts with all alpha V integrins.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB235650

Human CD51 ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style
left-column

製品の詳細

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.
Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGAV
style
left-column

文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111511 PubMed36261010

2022

A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Ghochani,Sree Deepthi Muthukrishnan,Alireza Sohrabi,Riki Kawaguchi,Michael C Condro,Soniya Bastola,Fuying Gao,Yue Qin,Jack Mottahedeh,M Luisa Iruela-Arispe,Nagesh Rao,Dan R Laks,Linda M Liau,Gary W Mathern,Steven A Goldman,S Thomas Carmichael,Ichiro Nakano,Giovanni Coppola,Stephanie K Seidlits,Harley I Kornblum

Nanomaterials (Basel, Switzerland) 11: PubMed34947606

2021

Homing Peptide-Based Targeting of Tenascin-C and Fibronectin in Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Simón-Gracia,Kristina Kiisholts,Vilma Petrikaitė,Allan Tobi,Merli Saare,Prakash Lingasamy,Maire Peters,Andres Salumets,Tambet Teesalu

Journal of materials chemistry. B 9:7205-7215 PubMed33710248

2021

Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Assunção,Chi Him Kendrick Yiu,Ho-Ying Wan,Dan Wang,Dai Fei Elmer Ker,Rocky S Tuan,Anna Blocki

Scientific reports 11:11328 PubMed34059712

2021

Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan W J Steel,Vladimir Vigdorovich,Nicholas Dambrauskas,Brandon K Wilder,Silvia A Arredondo,Debashree Goswami,Sudhir Kumar,Sara Carbonetti,Kristian E Swearingen,Thao Nguyen,Will Betz,Nelly Camargo,Bridget S Fisher,Jo Soden,Helen Thomas,Jim Freeth,Robert L Moritz,D Noah Sather,Stefan H I Kappe

Molecular oncology 15:1543-1565 PubMed33605506

2021

EWI-2 controls nucleocytoplasmic shuttling of EGFR signaling molecules and miRNA sorting in exosomes to inhibit prostate cancer cell metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenying Fu,Qing Zhang,Ani Wang,Songpeng Yang,Yangfu Jiang,Lin Bai,Quan Wei

Cancers 13: PubMed33807786

2021

Integrin αvβ3 Engagement Regulates Glucose Metabolism and Migration through Focal Adhesion Kinase (FAK) and Protein Arginine Methyltransferase 5 (PRMT5) in Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pulin Che,Lei Yu,Gregory K Friedman,Meimei Wang,Xiaoxue Ke,Huafeng Wang,Wenbin Zhang,Burt Nabors,Qiang Ding,Xiaosi Han

Nature communications 12:1140 PubMed33602902

2021

Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Castro-Córdova,Paola Mora-Uribe,Rodrigo Reyes-Ramírez,Glenda Cofré-Araneda,Josué Orozco-Aguilar,Christian Brito-Silva,María José Mendoza-León,Sarah A Kuehne,Nigel P Minton,Marjorie Pizarro-Guajardo,Daniel Paredes-Sabja

Theranostics 10:231-246 PubMed31903117

2020

Soluble CD146, a cerebrospinal fluid marker for neuroinflammation, promotes blood-brain barrier dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Daji Wang,Hongxia Duan,Jing Feng,Jianquan Xiang,Liqun Feng,Dan Liu,Xuehui Chen,Lin Jing,Zheng Liu,Dexi Zhang,Hongjun Hao,Xiyun Yan

Molecular pharmaceutics 16:292-304 PubMed30452273

2018

Cluster of Differentiation 46 Is the Major Receptor in Human Blood-Brain Barrier Endothelial Cells for Uptake of Exosomes Derived from Brain-Metastatic Melanoma Cells (SK-Mel-28).

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Kuroda,Masanori Tachikawa,Yuta Yagi,Mina Umetsu,Armania Nurdin,Eisuke Miyauchi,Michitoshi Watanabe,Yasuo Uchida,Tetsuya Terasaki

Journal of cell science 131: PubMed29361527

2018

α5β1 integrin trafficking and Rac activation are regulated by APPL1 in a Rab5-dependent manner to inhibit cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole L Diggins,Hakmook Kang,Alissa Weaver,Donna J Webb
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB211845

PE Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

pe-integrin-alpha-v-antibody-epr16800-ab211845

0

(0 reviews)

Alternative Product
Primary Antibodies

AB207286

Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-594-integrin-alpha-v-antibody-epr16800-ab207286

0

(0 reviews)

Alternative Product
Primary Antibodies

AB204684

Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-647-integrin-alpha-v-antibody-epr16800-ab204684

0

(0 reviews)

Alternative Product
Primary Antibodies

AB208012

Anti-Integrin alpha V antibody [EPR19669]

primary-antibodies

integrin-alpha-v-antibody-epr19669-ab208012

0

(0 reviews)

Alternative Product
Primary Antibodies

AB222222

Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free

primary-antibodies

integrin-alpha-v-antibody-epr16800-low-endotoxin-azide-free-ab222222

0

(0 reviews)

Alternative Product
Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

integrin-alpha-v-antibody-epr16800-ab179475

5

(10 reviews)

Alternative Product
Primary Antibodies

AB124968

Anti-Integrin alpha V antibody [EPR5583]

primary-antibodies

integrin-alpha-v-antibody-epr5583-ab124968

4

(2 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com