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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Integrin alpha 6 抗体 [EPR18124] (ab181551)

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Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
  • Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18124] to Integrin alpha 6
  • Suitable for: WB, IHC-P, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

製品の概要

  • 製品名

    Anti-Integrin alpha 6 antibody [EPR18124]
    Integrin alpha 6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18124] to Integrin alpha 6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SW480, A549, F6 whole cell lysates; Mouse skin lysate and Rat skin lysate, Human fetal kidney tissue lysate, Mouse kidney and spleen tissue lysate, Rat spleen tissue lysate. IHC-P: Human breast tissue, Human endometrial cancer tissue, Mouse stomach tissue, Rat stomach tissue. IHC-Fr: Rat and mouse stomach tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181551の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
IHC-P (3)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr (1)
1/100.
特記事項
WB
1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

ターゲット情報

  • 機能

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).

  • 組織特異性

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

  • 関連疾患

    Epidermolysis bullosa letalis, with pyloric atresia

  • 配列類似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • 翻訳後修飾

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.

  • 細胞内局在

    Cell membrane. Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alpha6p antibody
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • Processed integrin alpha-6 antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    画像

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution

      Lane 1 : Wild-type SW480 cell lysate
      Lane 2 : ITGA6 knockout SW480 cell lysate
      Lane 3 : HEK-293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 127 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Lanes 1 - 3: Merged signal (red and green). Green - ab181551 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181551 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemistry (Frozen sections) analysis of rat stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI. 

      Positive staining was seen on rat stomach.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.  

      Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

      Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma) whole cell lysate
      Lane 3 : Mouse skin lysate
      Lane 4 : Rat skin lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Frozen sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI. 

      Positive staining was seen on mouse stomach.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Human endometrial cancer  tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Basement membrane staining on tumor cells of Rat stomach tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 10 seconds


      Blocking and diluting buffer was 5% NFDM /TBST

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

      Lane 1 : F9 (mouse embryo testicular cancer cell line) whole cell lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Basement membrane staining on tumor cells of Mouse stomach tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

    参考文献 (39)

    ab181551 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181551 は 39 報の論文で使用されています。

    • Yang H  et al. Analysis of miR-203a-3p/SOCS3-mediated induction of M2 macrophage polarization to promote diabetic wound healing based on epidermal stem cell-derived exosomes. Diabetes Res Clin Pract 197:110573 (2023). PubMed: 36764461
    • Lawaetz M  et al. Potential of uPAR, αvβ6 Integrin, and Tissue Factor as Targets for Molecular Imaging of Oral Squamous Cell Carcinoma: Evaluation of Nine Targets in Primary Tumors and Metastases by Immunohistochemistry. Int J Mol Sci 24:N/A (2023). PubMed: 36835265
    • Ma C  et al. Comprehensive analysis of autophagy-related gene expression profiles identified five gene biomarkers associated with immune infiltration and advanced plaques in carotid atherosclerosis. Orphanet J Rare Dis 18:66 (2023). PubMed: 36959587
    • Lu JH  et al. Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat. Int J Mol Sci 24:N/A (2023). PubMed: 37175945
    • Almeida SFF  et al. Unveiling the role of osteosarcoma-derived secretome in premetastatic lung remodelling. J Exp Clin Cancer Res 42:328 (2023). PubMed: 38031171
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    20 µg
    Treatment
    2 ug of Integrin alpha 6 contained plasmid were transfected for 24hrs
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 23 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human skin (foreskin))
    Permeabilization
    No
    Specification
    Human skin (foreskin)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Unfixed
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human foreskin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 8.4
    Permeabilization
    No
    Specification
    Human foreskin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 31 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 23 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Rectum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    No
    Specification
    Rectum
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 30 2016

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