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AB226816

Anti-Integrin alpha 5 抗体

Anti-Integrin alpha 5 antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Integrin alpha 5 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGA5.

別名を表示する

CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5

3 Images
Western blot - Anti-Integrin alpha 5 antibody (AB226816)
  • WB

Supplier Data

Western blot - Anti-Integrin alpha 5 antibody (AB226816)

All lanes:

Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 30 µg

Lane 2:

MDA-MB-231 membrane extract lysate at 30 µg

Predicted band size: 115 kDa,47 kDa

true

Western blot - Anti-Integrin alpha 5 antibody (AB226816)
  • WB

Supplier Data

Western blot - Anti-Integrin alpha 5 antibody (AB226816)

All lanes:

Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution

All lanes:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 115 kDa

true

Western blot - Anti-Integrin alpha 5 antibody (AB226816)
  • WB

Supplier Data

Western blot - Anti-Integrin alpha 5 antibody (AB226816)

All lanes:

Western blot - Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 115 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

Recombinant Fragment Protein within Human ITGA5. The exact immunogen used to generate this antibody is proprietary information.

P08648

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代替製品

Primary Antibodies

AB150361

Anti-Integrin alpha 5 antibody [EPR7854]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Integrin alpha 5 antibody [EPR7854] (AB150361)

  • 5
  • 13
Primary Antibodies

AB221606

Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - Low endotoxin, Azide free (AB221606)

  • 0
  • 0
Primary Antibodies

AB239400

Anti-Integrin alpha 5 antibody [EPR7854] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 5 antibody [EPR7854] - BSA and Azide free (AB239400)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB179471

Anti-Integrin beta 1 antibody [EPR16895]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] (AB179471)

  • 5
  • 5
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

  • 5
  • 10
Primary Antibodies

AB309092

Anti-Integrin beta 5 antibody [EPR26930-21]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.
Biological function summary

Integrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.

Pathways

Integrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.

Integrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (PubMed : 33962943). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed : 29030430). ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed : 31331973).. (Microbial infection) Integrin ITGA5 : ITGB1 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human parvovirus B19.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGA5
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical science (London, England : 1979) 138:251-268 PubMed38362910

2024

Inhibition of the histone methyltransferase EZH2 induces vascular stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Ibarrola,Rachel R Xiang,Zhe Sun,Qing Lu,Michael A Hill,Iris Z Jaffe

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

OncoTargets and therapy 13:5323-5335 PubMed32606750

2020

Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting β-Tubulin in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Fu,Xiaoyin Sun,Zhangyuan Gu,Zhigang Zhuang
View all publications
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