Anti-Integrin alpha 3 抗体 [J143] (ab11767)
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 3 antibody [J143] (ab11767)](https://www.abcam.co.jp/ps/products/11/ab11767/Images/ab11767-19079-anti-integrin-alpha-3-antibody-j143-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 3 antibody [J143] (ab11767)")
Key features and details
- Mouse monoclonal [J143] to Integrin alpha 3
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Integrin alpha 3 antibody [J143]
Integrin alpha 3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [J143] to Integrin alpha 3 -
由来種
Mouse -
特異性
This antibody recognizes the alpha 3 subunit of VLA3 (ECMRI - extracellular matrix receptor I, URO1), which has been shown to be related to the chicken fibronectin/laminin receptor. VLA proteins are part of the integrin family of cell adhesion molecules, and the distribution of VLA3 in cells and tissues suggests a possible relationship with cellular adhesion. This antibody detects glycoproteins of 140, 120 and 30kDa. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin alpha 3. developed using the 253J Bladder cancer cell line
Database link: P26006 -
ポジティブ･コントロール
- Kidney.
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特記事項
The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
一次抗体 備考
The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression. -
ポリ/モノ
モノクローナル -
クローン名
J143 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11767の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/40.
|
| 特記事項 |
|---|
|
ICC/IF
1/40. |
ターゲット情報
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機能
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. -
組織特異性
Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻訳後修飾
Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3675 Human
- Omim: 605025 Human
- SwissProt: P26006 Human
- Unigene: 265829 Human
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別名
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 3 antibody [J143] (ab11767)ICC/IF image of ab11767 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11767, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab11767 は 4 報の論文で使用されています。
- Anandi L et al. DNA-dependent protein kinase plays a central role in transformation of breast epithelial cells following alkylation damage. J Cell Sci 130:3749-3763 (2017). PubMed: 28923836
- Cagnet S et al. Signaling events mediated by a3ß1 integrin are essential for mammary tumorigenesis. Oncogene N/A:N/A (2013). PubMed: 24077284
- McKeen Polizzotti L et al. Novel Image Analysis Approach Quantifies Morphological Characteristics of 3D Breast Culture Acini with Varying Metastatic Potentials. J Biomed Biotechnol 2012:102036 (2012). ICC/IF ; Human . PubMed: 22665978
- Van den Bergh F et al. Type XVII collagen (BP180) can function as a cell-matrix adhesion molecule via binding to laminin 332. Matrix Biol 30:100-8 (2011). PubMed: 21034821