Anti-Insulin Receptor beta 抗体 [C18C4] (ab69508)

According to the Uniprot homepage the alpha chain is located from amino acid 28-758, whereas the beta chain is from aa 763-1382:

http://www.uniprot.org/uniprot/P06213

ab69508 has its immunogen in the cytoplasmic domain of the beta subunit; this sequence seen here http://www.uniprot.org/uniprot/P06213#PRO_0000016687 as aa 980-1382 is buried in the beta subunit and does not fall in the alpha subunit which is aa 28-758 so I would not expect cross reactivity with alpha in this case. This has not been experimentally tested.

https://www.abcam.com/Insulin-Receptor-beta-antibody-C18C4-ab69508.html (or use the following: https://www.abcam.com/Insulin-Receptor-beta-antibody-C18C4-ab69508.html).

ab983 : I am happy to confirm that product ab983 is specific for the β-subunit of human insulin receptor. This was determined by (1) immunoblotting on human insulin receptor and (2) epitope analysis of short receptor peptides. The minimum epitope for this antibody was defined as a sequence PEEHRP corresponding to amino acids 765-770 close to the N-terminus of the β-subunit.

https://www.abcam.com/Insulin-Receptor-beta-antibody-18-44-ab983.html (or use the following: https://www.abcam.com/Insulin-Receptor-beta-antibody-18-44-ab983.html).

ab80527 : This antibody was generated using synthetic peptide corresponding the aa Tyr-KKNGRILTLPRSNPS from the C-terminal of human insulin receptor B-subunit. And therefore is also expected to be specific for the beta chain.

https://www.abcam.com/Insulin-Receptor-beta-antibody-CT-1-ab80527.html (or use the following: https://www.abcam.com/Insulin-Receptor-beta-antibody-CT-1-ab80527.html).

More information on these antibodies can be found on their respective datasheets by following the links.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Thank you for your enquiry. I am sorry to hear this vial of ab52167 is giving you trouble. We don't have a specificIHC-P protocol available for this antibody. But If you could, please complete the formbelow so I can get an idea of your protocol and data. This will make it easier for me to offer troubleshooting tips. If the antibody continues toperform poorly after taking into account anytips, I would behappyto offer a replacement, credit or refund.

Thanks in advance for the additional information!

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1) Abcam product code ab



2) Abcam order reference number or product batch number



3) Description of the problem



4) Sample preparation:

Species

Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other:

Sample preparation

Positive control

Negative control



5) Fixation step

Yes/No

If yes: Fixative agent and concentration

Fixation time

Fixation temperature



6) Antigen retrieval method



7) Permeabilization method:

Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?

Permeabilizing agent and concentration:





8) Blocking agent (eg BSA, serum…):

Concentration

Blocking time

Blocking temperature



9) Endogenous peroxidases blocked?

Endogenous biotins blocked?



10) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

Diluent buffer

Incubation time



11) Secondary antibody:

Species:

Reacts against:

Concentration or dilution

Diluent buffer

Incubation time

Fluorochrome or enzyme conjugate



12) Washing after primary and secondary antibodies:

Buffer

Number of washes



13) Detection method



14) How many times have you run this staining?

Do you obtain the same results every time?

What steps have you altered to try and optimize the use of this antibody?



Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.





Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps testé en IHC-P tel que ab28455 (www.abcam.com/ab28455) ou ab115693 (www.abcam.com/ab115693). Concernant les deux anti-PCK1, à ma connaissance ces anticorps n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab28455 ou ab115693 chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab28455 ou ab115693 pourra être utilisable lors d’une prochaine commande d’anticorps primaire. Etapes à suivre : 1. Nous confirmer que vous souhaiteriez tester ab28455 ou ab115693 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab28455 ou ab115693. 2. Acheter ab28455 ou ab115693 par téléphone, fax ou internet (www.abcam.com) 3. Le tester chez la souris 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si ab28455 ou ab115693 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.