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AB8305

Anti-Insulin + Proinsulin 抗体 [D3E7]

Anti-Insulin + Proinsulin antibody [D3E7]

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(2 Publications)

Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody detecting Insulin + Proinsulin in IHC-Fr, ELISA. Suitable for Cow, Human, Mouse, Pig, Rat.

- Trusted since 2002

別名を表示する

Insulin, INS

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

D3E7

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse, Rat, Cow, Human, Pig

アプリケーション

IHC-Fr, ELISA

applications

特異性

Kd for this antibody is 6.3 x 10-8M.This antibody is specific for both insulin and proinsulin

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), ELISA in Cow, Human, Mouse, Pig, Rat samples.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.
Biological function summary

Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.

Pathways

Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.

Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36142497

2022

Recombinant Reg3α Prevents Islet β-Cell Apoptosis and Promotes β-Cell Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Luting Yu,Liang Li,Junli Liu,Hao Sun,Xiang Li,Hanyu Xiao,Martin Omondi Alfred,Min Wang,Xuri Wu,Yan Gao,Chen Luo

Gastroenterology 141:1451-62, 1462.e1-6 PubMed21763240

2011

Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice.

Applications

IHC-Fr

Species

Mouse

Angela Criscimanna,Julie A Speicher,Golbahar Houshmand,Chiyo Shiota,Krishna Prasadan,Baoan Ji,Craig D Logsdon,George K Gittes,Farzad Esni
View all publications
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代替製品

Primary Antibodies

AB8304

Anti-Insulin + Proinsulin antibody [D6C4]

  • 4
  • 6

組合せ製品

Assay Kits

AB242235

Human Proinsulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)

  • 0
  • 0
Primary Antibodies

AB92517

Anti-Glucagon antibody [EP3070]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)

  • 5
  • 8
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

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