Anti-Insulin 抗体 [E2E3] (ab9569)
Key features and details
- Mouse monoclonal [E2E3] to Insulin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Insulin antibody [E2E3]
Insulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [E2E3] to Insulin -
由来種
Mouse -
特異性
The antibody recognizes the biologically most active forms of insulin on the C terminal end. The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method). -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Pig Insulin. Full length Native protein
Database link: P01315 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E2E3 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Affibody® Molecule
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100.
Staining of formalin-fixed tissue sections requirestreating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT. |
特記事項 |
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IHC-P
1/100. Staining of formalin-fixed tissue sections requirestreating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT. |
ターゲット情報
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機能
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. -
関連疾患
Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
- Unigene: 272259 Human
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別名
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)Human pancreas stained with ab9569.The antibody labels the cytoplasm of ß cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)Image courtesy of an abreview from Nizar Mourad.
Formalin-fixed, paraffin-embedded monkey pancreas tissue stained for Insulin using ab9569 at 1/800 dilution in immunhistochemical analysis.
Goat Ani-mouse HRP was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab9569 は 5 報の論文で使用されています。
- Guruswamy Damodaran R & Vermette P Decellularized pancreas as a native extracellular matrix scaffold for pancreatic islet seeding and culture. J Tissue Eng Regen Med 12:1230-1237 (2018). PubMed: 29499099
- Enderami SE et al. Generation of Insulin-Producing Cells From Human-Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet-Rich Plasma. J Cell Physiol 232:2878-2886 (2017). PubMed: 27925205
- Tian M et al. Alternative immunomodulatory strategies for xenotransplantation: CD80/CD86-CTLA4 pathway-modified immature dendritic cells promote xenograft survival. PLoS One 8:e69640 (2013). IHC-P . PubMed: 23922766
- Zhai C et al. Porcine CTLA4-Ig prolong islet xenografts in rats by downregulating the direct pathway of T-cell activation. Xenotransplantation 18:40-5 (2011). PubMed: 21342286
- Nykiforuk CL et al. Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds. Plant Biotechnol J 4:77-85 (2006). WB ; Human . PubMed: 17177787