AB210560

Anti-Insulin 抗体

Anti-Insulin antibody

Lab Essentials
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(10 Publications)

Rabbit Polyclonal Insulin antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish ins aa 1 to C-terminus.

別名を表示する

Insulin, ins

2 Images

IHC - Wholemount - Anti-Insulin antibody (AB210560)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Insulin antibody (AB210560)

Immunohistochemistry - Whole mount of Zebrafish embryo labeling Insulin in endocrine β-cells with ab210560 at a 1/200 dilution.

IHC - Wmt

Unknown

IHC - Wholemount - Anti-Insulin antibody (AB210560)

Immunohistochemistry - whole mount of Zebrafish embryo 3 days post fertilisation labeling Insulin with ab210560 at a 1/200 dilution.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Zebrafish

アプリケーション

IHC - Wmt

applications

免疫原

Recombinant Fragment Protein within Zebrafish ins aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O73727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information ins

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7199 PubMed40789835

2025

A continuously oxygenated macroencapsulation system enables high-density packing and delivery of insulin-secreting cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tung T Pham,Phuong L Tran,Linda A Tempelman,Simon G Stone,Christopher Piccirillo,Alan Li,James A Flanders,Minglin Ma

Diabetologia 68:1754-1768 PubMed40295334

2025

Pancreatic exocrine damage induces beta cell stress in zebrafish larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Noura Faraj,Willem M H Hoogaars,B H Peter Duinkerken,Anouk H G Wolters,Kim Kats,Mette C Dekkers,Arnaud Zaldumbide,Ben N G Giepmans

Diabetic medicine : a journal of the British Diabetic Association 41:e15279 PubMed38185936

2024

Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xu,Yunting Zhou,Tianyuan Wang,Chengming Ni,Chunlei Wang,Rui Li,Xuekui Liu,Jun Liang,Tzu-Wen Hong,Bo Liu,Aileen J F King,Shanta J Persaud,Zilin Sun,Peter M Jones

Development (Cambridge, England) 150: PubMed36897579

2023

Pax4-Ghrelin mediates the conversion of pancreatic ε-cells to β-cells after extreme β-cell loss in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Junqin Yu,Jianlong Ma,Yanfeng Li,Yang Zhou,Lingfei Luo,Yun Yang

Frontiers in molecular biosciences 8:822232 PubMed35127826

2022

Integrated Array Tomography for 3D Correlative Light and Electron Microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan Lane,Anouk H G Wolters,Ben N G Giepmans,Jacob P Hoogenboom

Anatomia, histologia, embryologia 50:594-603 PubMed33660337

2021

Unique localization of disseminated pancreas in the oesophagus of catfish (clarias gariepinus) with reference to sexual dimorphism.

Applications

Unspecified application

Species

Unspecified reactive species

Mayada W Karkit,Hoda F Salem,Mohammad H Bareedy,Yaser H A Elewa

Journal of molecular endocrinology 66:129-140 PubMed33350979

2020

The dual role of RFX6 in directing β cell development and insulin production.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Lu,Cheng Cheng,Zhen-Chao Cheng,Qian Wu,Han Shen,Ming-Xia Yuan,Bo Zhang,Jin-Kui Yang

Diabetes 69:2794-2805 PubMed32912862

2020

In Vivo Reporter Assays Uncover Changes in Enhancer Activity Caused by Type 2 Diabetes-Associated Single Nucleotide Polymorphisms.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Eufrásio,Chiara Perrod,Fábio J Ferreira,Marta Duque,Mafalda Galhardo,José Bessa

Cell reports 32:107862 PubMed32640228

2020

A Conserved Notochord Enhancer Controls Pancreas Development in Vertebrates.

Applications

Unspecified application

Species

Unspecified reactive species

João Pedro Amorim,Ana Gali-Macedo,Hugo Marcelino,Renata Bordeira-Carriço,Silvia Naranjo,Solangel Rivero-Gil,Joana Teixeira,Mafalda Galhardo,Joana Marques,José Bessa

European journal of histochemistry : EJH 63: PubMed30827082

2019

Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae).

Applications

Unspecified application

Species

Unspecified reactive species

Burak Kaptaner

View all publications

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