Anti-Insulin + Proinsulin 抗体 [D6C4] (ab8304)

Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même n’a pas été testé chez le rat mais il y’a des chances que cela fonctionne. Si vous souhaitez le tester, je peux vous faire une offre de test.

I am very pleased to hear you would like to use ab8304 in WB and send us an image of your results.

This code will give you $367 off your next order before the expiration date. To redeem this offer, please submit an Abreview with the image for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Thank you for contacting us. I found in our records a note stating that western blotting was added to the list of tested applications for the insulin/pro-insulin antibody ab8304 based on a single report from a customer. Unfortunately, the customer did not send an image of their blot. They briefly described their protocol as follows:

Dilution: 1/2000. Use under non reducing condition. A customer reported successfully staining a nitrocellulose blot of mammalian cell culture media transferred from a non-reducing SDS-PAGE gel. The blot was blocked with 4% skim milk in PBS.

Please let me know if you have any questions.

Thank you for your enquiry.



Please find enclosed the requested documents as attachments.



If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
https://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
https://www.abcam.com/insulin-proinsulin-antibody-d3e7-biotin-ab20756.html
We have not specifically tested human analogs with this kit but based on sequence similarity, we would expect the kit to recognize these as well.  However, we wouldn't guarantee it since it hasn't been specifically tested.
I hope this information helps. Please contact us with any other questions.

Thank you for your patience. My colleague has just added the biotinylated form of this clone to our catalog as ab133958. I hope this helps, please let me know if you need any additional assistance.

Thank you for contacting us.

While we do not currently have a biotin conjugated form of ab8304 in our catalog, I am looking into the possibility of adding one for you. I will let you know as soon as I have any information about whether this will be possible or not. We do have another Insulin + Proinsulin antibody that is already biotinylated: ab20756. Alternatively, if you would like to biotin conjugated ab8304, we do sell conjugation kits that would be suitable for that purpose, such as ab102867. I hope this helps, please let me know if you need any additional information or assistance and I will be happy to help you further.