Anti-iNOS 抗体 [SP126] (ab115819)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-5-AntiiNOS-antibody-SP126-IHC-Human-lung-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP126] to iNOS
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-iNOS antibody [SP126]
iNOS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP126] to iNOS -
由来種
Rabbit -
特異性
Not suitable for mouse IHC-P.
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アプリケーション
適用あり: IHC-P, Flow Cyt (Intra)more details
適用なし: ELISA or WB -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human lung and lung carcinoma tissue. Flow Cyt (Intra): RAW 264.7 cells.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
特記事項（精製）
Purified from TCS by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP126 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
- Alexa Fluor® 568 Anti-iNOS antibody [SP126] (ab312935)
- Anti-pan NOS antibody [RM1126] (ab316334)
- Anti-pan NOS antibody [RM1126] - BSA and Azide free (ab316335)
- Alexa Fluor® 488 Anti-iNOS antibody [SP126] (ab317527)
- Alexa Fluor® 750 Anti-iNOS antibody [SP126] (ab320971)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115819の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
1/100.
Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-P
1/100. Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
組織特異性
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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参照データベース
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 709191 Human
see all -
別名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).
Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.
Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-4-AntiiNOS-antibody-SP126-IHC-Human-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).
Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.
Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
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![Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-330589-anti-inos-antibody-sp126-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] (ab115819)Flow cytometry analysis of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 0.1ug/ml Lipopolysaccharides for 24hours labeling iNOS with purified ab115819 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-301171-anti-inos-antibody-sp126-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-127968-anti-inos-antibody-sp126-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.
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![Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.co.jp/ps/products/115/ab115819/Images/ab115819-6-benefits-of-recombinant-antibodies.png)
Anti-iNOS antibody [SP126] (ab115819)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab115819 は 22 報の論文で使用されています。
- Owembabazi E et al. Co-administration of alcohol and combination antiretroviral therapy (cART) in male Sprague Dawley rats: A study on testicular morphology, oxidative and cytokines perturbations. Anat Cell Biol 56:236-251 (2023). PubMed: 36759974
- Ding J et al. Identification of an Autophagy-Related Signature for Prognosis and Immunotherapy Response Prediction in Ovarian Cancer. Biomolecules 13:N/A (2023). PubMed: 36830707
- Huang A et al. Bardoxolone Methyl Ameliorates Myocardial Ischemia/Reperfusion Injury by Activating the Nrf2/HO-1 Signaling Pathway. Cardiovasc Ther 2023:5693732 (2023). PubMed: 36874247
- Tang Q et al. Embelin attenuates lipopolysaccharide-induced acute kidney injury through the inhibition of M1 macrophage activation and NF-κB signaling in mice. Heliyon 9:e14006 (2023). PubMed: 36938407
- Liu S et al. Multi-organ landscape of therapy-resistant melanoma. Nat Med 29:1123-1134 (2023). PubMed: 37106167