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AB136918

Anti-iNOS 抗体 [K13-A]

Anti-iNOS antibody [K13-A]

4

(11 Reviews)

|

(20 Publications)

Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.

別名を表示する

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

3 Images
Western blot - Anti-iNOS antibody [K13-A] (AB136918)
  • WB

Lab

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

Lane 1:

Raw264.7 Whole Cell Lysate at 20 µg

Lane 2:

Raw264.7 Whole Cell Lysate (LPS stimulated) at 20 µg

Secondary

All lanes:

Goat anit Rabbit IR680 at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 140 kDa,15 kDa,36 kDa,42 kDa,50 kDa

false

Western blot - Anti-iNOS antibody [K13-A] (AB136918)
  • WB

Lab

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

All lanes:

iNOS Overexpression Lysate (Human) at 0.1 µg

Secondary

All lanes:

Goat anti Rabbit IR680 at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 140 kDa

false

Western blot - Anti-iNOS antibody [K13-A] (AB136918)
  • WB

Unknown

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 131 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

K13-A

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human NOS2. The exact immunogen used to generate this antibody is proprietary information.

P35228

エピトープ

Antibody recognizes the epitope located between Ser1118 - Gly1129

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.</p>" } } }
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4

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こちらの製品も推奨する理由

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お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
バッファー組成
pH: 8 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.32% Tris HCl
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).
See full target information NOS2
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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37891965

2023

Ellagic Acid Prevented Dextran-Sodium-Sulfate-Induced Colitis, Liver, and Brain Injury through Gut Microbiome Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Ha Kim,Ji-Su Kim,Jae-Hee Kwon,In-Sook Kwun,Moon-Chang Baek,Gi-Seok Kwon,Wiramon Rungratanawanich,Byoung-Joon Song,Do-Kyun Kim,Hyo-Jung Kwon,Young-Eun Cho

International journal of molecular sciences 24: PubMed37569348

2023

Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Na Song,Jae-Won Lee,Hyung Won Ryu,Jae Kyoung Lee,Eun Sol Oh,Doo-Young Kim,Hyunju Ro,Dahye Yoon,Ji-Yoon Park,Sung-Tae Hong,Mun-Ock Kim,Su Ui Lee,Dae Young Lee

Frontiers in pharmacology 13:1075746 PubMed36532726

2022

Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Annamaria Fidilio,Margherita Grasso,Giuseppe Caruso,Nicolò Musso,Veronica Begni,Anna Privitera,Sebastiano Alfio Torrisi,Patrizia Campolongo,Stefania Schiavone,Fabio Tascedda,Gian Marco Leggio,Filippo Drago,Marco Andrea Riva,Filippo Caraci

Cancer genomics & proteomics 19:727-739 PubMed36316038

2022

Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed E M Saeed,Candela Cives-Losada,Thomas Efferth

Inflammopharmacology 30:2197-2209 PubMed35948811

2022

Nebivolol elicits a neuroprotective effect in the cuprizone model of multiple sclerosis in mice: emphasis on M1/M2 polarization and inhibition of NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Antoinette G Naeem,Reem N El-Naga,Haidy E Michel

Frontiers in pharmacology 12:809541 PubMed35002742

2021

Antioxidant Activity of Fluoxetine and Vortioxetine in a Non-Transgenic Animal Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Caruso,Margherita Grasso,Annamaria Fidilio,Sebastiano Alfio Torrisi,Nicolò Musso,Federica Geraci,Maria Rosaria Tropea,Anna Privitera,Fabio Tascedda,Daniela Puzzo,Salvatore Salomone,Filippo Drago,Gian Marco Leggio,Filippo Caraci

Dose-response : a publication of International Hormesis Society 19:15593258211044329 PubMed34690616

2021

SR-5, the specific ratio of Korean multi-herbal formula: An evaluation of antiulcerogenic effects on experimentally induced gastric ulcers in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kyeong Jo Kim,Eun Kim,Wan Seok Kang,Mijin Jeon,Hakjoon Choi,Ki Hoon Lee,Mi-Hyeon Kim,Jin Seok Kim,Chang-Su Na,Sunoh Kim

Frontiers in pharmacology 12:661072 PubMed34122080

2021

Rhoifolin Ameliorates Osteoarthritis Regulating Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyuan Yan,Bowei Ni,Gaohong Sheng,Yingchi Zhang,Yifan Xiao,Yongzhuang Ma,Hao Li,Hua Wu,Chang Tu

Frontiers in cell and developmental biology 8:572689 PubMed33330451

2020

Exosomal miRNA-16-5p Derived From M1 Macrophages Enhances T Cell-Dependent Immune Response by Regulating PD-L1 in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtian Li,Bing Suo,Gang Long,Yue Gao,Jia Song,Mengzhe Zhang,Baiyu Feng,Ce Shang,Dawei Wang

Biochimica et biophysica acta. Bioenergetics 1861:148290 PubMed32828729

2020

Modulation of peroxynitrite produced via mitochondrial nitric oxide synthesis during Ca and succinate-induced oxidative stress in cardiac isolated mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Harrison J Gerdes,Meiying Yang,James S Heisner,Amadou K S Camara,David F Stowe
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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