Anti-INO80C 抗体 (ab151046)
Key features and details
- Rabbit polyclonal to INO80C
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-INO80C antibody -
製品の詳細
Rabbit polyclonal to INO80C -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details
適用なし: WB -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment, corresponding to a sequence from Human INO80C.
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ポジティブ･コントロール
- ICC: U-2 OS whole cells; IHC-P: Human kidney, testits and skeletal muscle tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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関連性
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 125476 Human
- Entrez Gene: 291737 Rat
- SwissProt: Q6PI98 Human
- SwissProt: Q5BJY3 Rat
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別名
- C18orf37 antibody
- FLJ38183 antibody
- hIes6 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)
Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling INO80C at the nucleoli fibrilar center with ab151046 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human kidney tissue labeling INO80C of tubules cells with ab151046 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human testis tissue labeling INO80C at the nuclues of seminiferous ducts with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human skeletal muscle tissue labeling INO80C of myocytes with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)This image is courtesy of an anonymous Abreviewab151046 staining INO80C in Mouse paraffin-fixed tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in Goat serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal (1/125) was used as the secondary antibody. Red - INO80C, Blue (DAPI) - nuclei.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab151046 は論文での使用が確認できていません。