Anti-INO80 抗体 (ab118787)
Key features and details
- Rabbit polyclonal to INO80
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-INO80 antibody
INO80 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to INO80 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
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免疫原
Synthetic peptide corresponding to a region within amino acids 1506-1556 of Human INO80 (NP_060023.1).
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ポジティブ･コントロール
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118787の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 176 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 176 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle. -
組織特異性
According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 DBINO domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The DBINO region is involved in binding to DNA. -
細胞内局在
Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 478262 Dog
- Entrez Gene: 54617 Human
- Entrez Gene: 100517567 Pig
- Entrez Gene: 100350680 Rabbit
- Omim: 610169 Human
- SwissProt: Q9ULG1 Human
- Unigene: 292949 Human
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別名
- DNA helicase INO80 antibody
- hINO80 antibody
- homolog of yeast INO80 antibody
see all
画像
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Western blot - Anti-INO80 antibody (ab118787)All lanes : Anti-INO80 antibody (ab118787) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 176 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-INO80 antibody (ab118787)Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab118787 は 13 報の論文で使用されています。
- Miura R et al. Chromatin remodeling factor, INO80, inhibits PMAIP1 in renal tubular cells via exchange of histone variant H2A.Z. for H2A. Sci Rep 13:13235 (2023). PubMed: 37580530
- Cheon Y et al. The chromatin remodeler Ino80 mediates RNAPII pausing site determination. Genome Biol 22:294 (2021). PubMed: 34663418
- Ka NL et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Cell Rep 37:110138 (2021). PubMed: 34936865
- Prendergast L et al. Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability. Nat Commun 11:4534 (2020). PubMed: 32913330
- Moreno-Andrés D et al. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 9:N/A (2020). PubMed: 32708675