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AB248247

Anti-Inhibin beta A 抗体 [EPR2785(2)] - BSA and Azide free

Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Inhibin beta A antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

別名を表示する

Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA

3 Images
Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)
  • WB

Unknown

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)

This data was developed using ab128958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (<a href='/products/primary-antibodies/inhibin-beta-a-antibody-epr27852-ab128958'>ab128958</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 43 kDa

false

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)
  • WB

Unknown

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)

This data was developed using ab128958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (<a href='/products/primary-antibodies/inhibin-beta-a-antibody-epr27852-ab128958'>ab128958</a>) at 1/1000 dilution

All lanes:

Human Inhibin beta A recombinant protein at 0.001 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

false

OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free (AB248247)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2785(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Proteins & Peptides

AB255808

Recombinant Human/Mouse/Rat Inhibin beta A protein (Active)

Bioactive

Functional Studies - Recombinant Human/Mouse/Rat Inhibin beta A protein (Active) (AB255808)

  • 0
  • 0

組合せ製品

Assay Kits

AB119568

Human Activin A ELISA Kit

Sandwich ELISA - Human Activin A ELISA Kit (AB119568)

  • 0
  • 0
Sample Prep & Detection Kits

AB102893

PE/Cy5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5® Conjugation Kit - Lightning-Link® (AB102893)

  • 0
  • 0
Sample Prep & Detection Kits

AB269892

Latex Conjugation Kit - 400nm Blue

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB40854

Anti-Smad3 antibody [EP568Y]

20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody [EP568Y] (AB40854)

  • 5
  • 10
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 7
Primary Antibodies

AB40855

Anti-Smad2 antibody [EP784Y]

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [EP784Y] (AB40855)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

ab248247 is the carrier-free version of ab128958.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin beta A also known as activin beta A chain and inhibin A is a protein with a significant role in the regulation of diverse physiological processes. This protein has a molecular mass of approximately 45 kDa and originates primarily from gonadal tissues but also from the placenta pituitary gland and other tissues. Inhibin beta A consists of several subunits that can dimerize to form both activins and inhibins depending on the pairing with other subunits like the alpha chain.
Biological function summary

Activin beta A chain plays an important role in the modulation of follicle-stimulating hormone (FSH) regulation cellular growth and differentiation. It often functions as part of a complex with activin and inhibin proteins influencing reproductive hormone levels and participating in tissue repair and inflammation processes. Inhibin beta A forms activin A when paired with another inhibin beta A subunit or inhibin A when paired with an inhibin alpha subunit therefore contributing to the modulation of FSH-releasing protein activity.

Pathways

Activin and inhibin proteins participate in the regulation of the TGF-beta signaling pathway which is essential for reproductive and developmental processes. In this context inhibin beta A interacts functionally with proteins such as follistatin and FSH-releasing protein which modulate pathways that influence follicle maturation and hormone secretion. The protein's role in these pathways highlights its importance in orchestrating hormonal responses and facilitating proper cellular communication within the endocrine system.

Inhibin beta A becomes relevant in the context of reproductive health and oncology. Changes in its expression can associate with disorders like polycystic ovary syndrome (PCOS) and certain cancers particularly ovarian cancer where it may alter hormonal regulation and tumor progression. Related proteins such as inhibin B and follistatin also play roles in these conditions further complicating the pathways and the potential development of therapeutic interventions that target inhibin beta A and its network of interactions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.. Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis (PubMed : 3194407, PubMed : 16440334). Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression (PubMed : 10652306, PubMed : 24018044). Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation (PubMed : 16440334). Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway (PubMed : 34948289). In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling (PubMed : 26333933).. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.
See full target information Inhibin beta A chain
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Abcam product promise

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