AB242422

Anti-Inhibin alpha 抗体 [SP323] - BSA and Azide free

Anti-Inhibin alpha antibody [SP323] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Inhibin alpha antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human samples.

別名を表示する

Inhibin alpha chain, INHA

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human testis tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach adenocarcinoma tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP323

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.</p>" } } }

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製品の詳細

ab242422 is the carrier-free version of ab224798.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Purified from TCS by protein A/G.

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin alpha also known as alpha-inhibin is a subunit of the inhibin protein complex. As a 32 kDa glycoprotein inhibin exerts its role as a modulator of hormone activities by forming heterodimers with various beta subunits typically expressed in the gonads adrenal glands and pituitary gland. Inhibin alpha not only influences the secretion of follicle-stimulating hormone (FSH) but also acts as an important player in reproductive endocrinology. Its expression can be assessed through inh alpha R1 and po12 markers offering insights through inhibin immunohistochemistry.

Biological function summary

Inhibin alpha forms part of the inhibin hormone complex which primarily serves as a negative regulator of FSH secretion. Insufficient levels of inhibin can disrupt normal metabolic functions impacting reproductive systems. The inhibin alpha subunit is vital for maintaining reproductive homeostasis and monitoring the inhibin staining pattern provides a useful diagnostic tool in reproductive health assessments. Inhibin binds to activin receptors where it blocks FSH release from the anterior pituitary gland reinforcing its role in managing the reproductive axis.

Pathways

Inhibin alpha is critically involved in the transforming growth factor-beta (TGF-beta) signaling pathway. This pathway plays a major role in cell differentiation and proliferation where inhibin competes with activin for the same receptors ultimately reducing FSH synthesis. Additionally inhibin functions within endocrine regulatory mechanisms ensuring the reproductive hormones maintain their balance alongside related proteins such as activin and follistatin each contributing uniquely to signaling processes.

Inhibin alpha alterations can significantly influence conditions like polycystic ovary syndrome (PCOS) and certain gonadal tumors. Abnormal levels of inhibin are often observed in PCOS where disrupted endocrine feedback leads to infertility issues. Gonadal tumors also relate to increased inhibin expression providing a biomarker for diagnosis and progression monitoring. Through these diseases inhibin alpha interacts with proteins such as luteinizing hormone playing part in their systemic dysfunctions within the affected individuals.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.. Inhibin B is a dimer of alpha and beta-B that plays a crucial role in the regulation of the reproductive system by inhibiting the secretion of follicle-stimulating hormone (FSH) from the anterior pituitary gland. Thereby, maintains reproductive homeostasis in both males and females. Acts as a more potent suppressor of FSH release than inhibin A (By similarity). Functions as competitive receptor antagonist binding activin type II receptors with high affinity in the presence of the TGF-beta type III coreceptor/TGFBR3L (PubMed : 34910520).

See full target information INHA

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Inhibin alpha 抗体 [SP323] - BSA and Azide free

Anti-Inhibin alpha antibody [SP323] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise