Anti-Inhibin alpha 抗体 [EPY2782] (ab81322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPY2782] to Inhibin alpha
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Inhibin alpha antibody [EPY2782]
Inhibin alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPY2782] to Inhibin alpha -
由来種
Rabbit -
特異性
Note: 20/23 kDa bands consist of the mature alpha chain. 50/53 kDa bands are the entire proprotein. -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Inhibin alpha aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P05111 -
ポジティブ・コントロール
- Human Adrenal gland, HeLa, Human placenta and LNCaP lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPY2782 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 40 kDa.
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特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 40 kDa. |
ターゲット情報
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機能
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
組織特異性
Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3623 Human
- Entrez Gene: 16322 Mouse
- Entrez Gene: 24504 Rat
- Omim: 147380 Human
- SwissProt: P05111 Human
- SwissProt: Q04997 Mouse
- SwissProt: P17490 Rat
- Unigene: 407506 Human
see all -
別名
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
see all
画像
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All lanes : Anti-Inhibin alpha antibody [EPY2782] (ab81322) at 1/2000 dilution
Lane 1 : Human Adrenal gland lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human placenta lysate
Lane 4 : LNCaP lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40,50 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Note: the 50 kDa band corresponds to the entire proprotein.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab81322 は 1 報の論文で使用されています。
- Han L et al. Characterization of the Mechanism of Inhibin a-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference. PLoS One 8:e74596 (2013). Mouse . PubMed: 24098340