AB47720

Anti-Inhibin alpha 抗体 [4A2F2]

Name: Anti-Inhibin alpha 抗体 [4A2F2] (ab47720) | Abcam
Brand: Abcam Limited
SKU: AB47720
Price: 72000 JPY
Availability: InStock

Anti-Inhibin alpha antibody [4A2F2]

Be the first to review this product! Submit a review

(6 Publications)

Mouse Monoclonal Inhibin alpha antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human INHA.

別名を表示する

Inhibin alpha chain, INHA

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunohistochemical analysis of paraffin-embedded human testicle tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunohistochemical analysis of paraffin-embedded human lymphoid tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

Flow Cytometry - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Flow Cyt

Unknown

Flow Cytometry - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Overlay histogram showing HeLa cells stained with ab47720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47720, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

ab47720 at 1/1000 dilution staining Inhibin alpha in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary show green staining in image while actin filaments have been labelled red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4A2F2

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human INHA. The exact immunogen used to generate this antibody is proprietary information.

P05111

特異性

This antibody is specific for Inhibin alpha.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified from tissue culture supernatant.

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin alpha also known as alpha-inhibin is a subunit of the inhibin protein complex. As a 32 kDa glycoprotein inhibin exerts its role as a modulator of hormone activities by forming heterodimers with various beta subunits typically expressed in the gonads adrenal glands and pituitary gland. Inhibin alpha not only influences the secretion of follicle-stimulating hormone (FSH) but also acts as an important player in reproductive endocrinology. Its expression can be assessed through inh alpha R1 and po12 markers offering insights through inhibin immunohistochemistry.

Biological function summary

Inhibin alpha forms part of the inhibin hormone complex which primarily serves as a negative regulator of FSH secretion. Insufficient levels of inhibin can disrupt normal metabolic functions impacting reproductive systems. The inhibin alpha subunit is vital for maintaining reproductive homeostasis and monitoring the inhibin staining pattern provides a useful diagnostic tool in reproductive health assessments. Inhibin binds to activin receptors where it blocks FSH release from the anterior pituitary gland reinforcing its role in managing the reproductive axis.

Pathways

Inhibin alpha is critically involved in the transforming growth factor-beta (TGF-beta) signaling pathway. This pathway plays a major role in cell differentiation and proliferation where inhibin competes with activin for the same receptors ultimately reducing FSH synthesis. Additionally inhibin functions within endocrine regulatory mechanisms ensuring the reproductive hormones maintain their balance alongside related proteins such as activin and follistatin each contributing uniquely to signaling processes.

Inhibin alpha alterations can significantly influence conditions like polycystic ovary syndrome (PCOS) and certain gonadal tumors. Abnormal levels of inhibin are often observed in PCOS where disrupted endocrine feedback leads to infertility issues. Gonadal tumors also relate to increased inhibin expression providing a biomarker for diagnosis and progression monitoring. Through these diseases inhibin alpha interacts with proteins such as luteinizing hormone playing part in their systemic dysfunctions within the affected individuals.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.. Inhibin B is a dimer of alpha and beta-B that plays a crucial role in the regulation of the reproductive system by inhibiting the secretion of follicle-stimulating hormone (FSH) from the anterior pituitary gland. Thereby, maintains reproductive homeostasis in both males and females. Acts as a more potent suppressor of FSH release than inhibin A (By similarity). Functions as competitive receptor antagonist binding activin type II receptors with high affinity in the presence of the TGF-beta type III coreceptor/TGFBR3L (PubMed : 34910520).

See full target information INHA

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:15508 PubMed37726363

2023

A 3D adrenocortical carcinoma tumor platform for preclinical modeling of drug response and matrix metalloproteinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priya H Dedhia,Hemamylammal Sivakumar,Marco A Rodriguez,Kylie G Nairon,Joshua M Zent,Xuguang Zheng,Katie Jones,Liudmila V Popova,Jennifer L Leight,Aleksander Skardal

Cancer medicine 10:1485-1500 PubMed33611864

2021

An immature inhibin-α-expressing subpopulation of ovarian clear cell carcinoma cells is related to an unfavorable prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Kusumoto,Masako Kurashige,Kenji Ohshima,Shinichiro Tahara,Takahiro Matsui,Satoshi Nojima,Satoshi Hattori,Eiichi Morii

Human reproduction (Oxford, England) 34:1019-1029 PubMed31119261

2019

Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hisashi Kataoka,Taisuke Mori,Hiroyuki Okimura,Hiroshi Matsushima,Fumitake Ito,Akemi Koshiba,Yukiko Tanaka,Kanoko Akiyama,Eiko Maeda,Takuya Sugahara,Yosuke Tarumi,Izumi Kusuki,Khaleque N Khan,Jo Kitawaki

Cancer research 78:2978-2989 PubMed29535220

2018

Inhibin Is a Novel Paracrine Factor for Tumor Angiogenesis and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Singh,Laura M Jenkins,Ben Horst,Victoria Alers,Shrikant Pradhan,Prabhjot Kaur,Tapasya Srivastava,Nadine Hempel,Balázs Győrffy,Eugenia V Broude,Nam Y Lee,Karthikeyan Mythreye

Medicine 96:e7998 PubMed28906379

2017

Accessory ovarian steroid cell tumor producing testosterone and cortisol: A case report.

Applications

IHC

Species

Human

Li Zang,Mingxia Ye,Guoqing Yang,Jinlong Li,Mei Liu,Jin Du,Weijun Gu,Nan Jin,Lijuan Yang,Jianming Ba,Jingtao Dou,Wensheng Fan,Yiming Mu,Yuanguang Meng,Zhaohui Lyu

PloS one 11:e0151640 PubMed26982590

2016

Shedding Light on the Nature of Seminal Round Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gianpiero D Palermo,Queenie V Neri,Tyler Cozzubbo,Stephanie Cheung,Nigel Pereira,Zev Rosenwaks

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Inhibin alpha 抗体 [4A2F2]

Anti-Inhibin alpha antibody [4A2F2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Flow Cytometry - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunocytochemistry/ Immunofluorescence - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [4A2F2] (AB47720)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise