Anti-ING4 抗体 [EP3804] (ab108621)
![Western blot - Anti-ING4 antibody [EP3804] (ab108621)](https://www.abcam.co.jp/ps/products/108/ab108621/Images/ab108621-483907-anti-ing4-antibody-ep3804-westernblot-human-mouse-rat.jpg "Western blot - Anti-ING4 antibody [EP3804] (ab108621)")
Key features and details
- Rabbit monoclonal [EP3804] to ING4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
-
製品名
Anti-ING4 antibody [EP3804]
ING4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3804] to ING4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197784) -
ポジティブ･コントロール
- WB: 293T, Raw264.7, and PC-12 cell lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP3804 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108621の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human ING4 peptide (ab197784).
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human ING4 peptide (ab197784). |
ターゲット情報
-
機能
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). -
配列類似性
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 51147 Human
- Entrez Gene: 28019 Mouse
- Entrez Gene: 297597 Rat
- Omim: 608524 Human
- SwissProt: Q9UNL4 Human
- SwissProt: Q8C0D7 Mouse
- Unigene: 524210 Human
- Unigene: 262547 Mouse
see all -
別名
- Brain my036 protein antibody
- Candidate tumor suppressor p33 ING 1 homolog antibody
- Candidate tumor suppressor p33 ING1 homolog antibody
see all
画像
-
Western blot - Anti-ING4 antibody [EP3804] (ab108621)All lanes : Anti-ING4 antibody [EP3804] (ab108621) at 1/1000 dilution (Purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab108621 は 4 報の論文で使用されています。
- Tang Y et al. ING4 Promotes Stemness Enrichment of Human Renal Cell Carcinoma Cells Through Inhibiting DUSP4 Expression to Activate the p38 MAPK/type I IFN-Stimulated Gene Signaling Pathway. Front Pharmacol 13:845097 (2022). PubMed: 35496267
- Lu L et al. Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1a and snail in HK2 cells. Acta Histochem 121:695-703 (2019). PubMed: 31239073
- Berger PL et al. Miz1, a Novel Target of ING4, Can Drive Prostate Luminal Epithelial Cell Differentiation. Prostate 77:49-59 (2017). PubMed: 27527891
- Pardo M et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). PubMed: 28811661