Anti-ING4 抗体 (ab113425)
Key features and details
- Rabbit polyclonal to ING4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ING4 antibody
ING4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ING4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
-
免疫原
Recombinant fragment, corresponding to internal sequence amino acids 33-183 of Human ING4, isoform 3 (BC007781).
-
ポジティブ･コントロール
- Human fetal liver, fetal kidney and Mouse kidney lysates; Human fetal brain tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Lyophilized:Reconstitute in 200ul sterile H2O. -
保存方法
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab113425 is purified by a protein affinity column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).
|
|
| IHC-P |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). |
|
IHC-P
1/100 - 1/500. |
ターゲット情報
-
機能
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). -
配列類似性
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 51147 Human
- Entrez Gene: 28019 Mouse
- Entrez Gene: 297597 Rat
- Omim: 608524 Human
- SwissProt: Q9UNL4 Human
- SwissProt: Q8C0D7 Mouse
- Unigene: 524210 Human
- Unigene: 262547 Mouse
see all -
別名
- Brain my036 protein antibody
- Candidate tumor suppressor p33 ING 1 homolog antibody
- Candidate tumor suppressor p33 ING1 homolog antibody
see all
画像
-
Western blot - Anti-ING4 antibody (ab113425)All lanes : Anti-ING4 antibody (ab113425) at 1/500 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Mouse kidney lysate
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ING4 antibody (ab113425)ab113425 at 1/100 dilution staining ING4 in Formalin-fixed, Paraffin-embedded Human fetal brain tissue by Immunohistochemistry. Note nuclear staining.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab113425 は 4 報の論文で使用されています。
- Ma T et al. Lentiviral vector with a radiation-inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells. Oncol Lett 21:67 (2021). PubMed: 33365078
- Tang X et al. miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/ß-catenin pathway. Oncol Lett 18:4621-4628 (2019). PubMed: 31611970
- Sun J et al. Inhibitor of growth 4 inhibits cell proliferation, migration, and induces apoptosis of renal cell carcinoma cells. J Cell Biochem N/A:N/A (2018). PubMed: 30390334
- Shao B & Liu E Expression of ING4 is negatively correlated with cellular proliferation and microvessel density in human glioma. Oncol Lett 14:3663-3668 (2017). PubMed: 28927128