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リコンビナントRabMAb

Anti-ING3 抗体 [EPR25273-6] (BSA and Azide free) (ab300082)

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Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
  • Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
  • Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
  • Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
  • Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
  • Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25273-6] to ING3 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-ING3 antibody [EPR25273-6] (BSA and Azide free)
    ING3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25273-6] to ING3 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate, HeLa transfected with siRNA specifically targeting ING3, whole cell lysate, HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, 293T (human embryonic kidney epithelial cell) whole cell lysate, NIH/3T3 (mouse embryonic fibroblast) whole cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.

  • 特記事項

    ab300082 is a carrier free version of ab300081. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300082の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 関連性

    Function: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Tissue specificity: Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Disease: Squamous cell carcinoma of the head and neck Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. Domain: The PHD-type zinc finger mediates the binding to H3K4me3.

  • 細胞内局在

    Nuclear

  • 参照データベース

    see all

  • 別名

    • 1300013A07Rik antibody
    • Eaf 4 antibody
    • Eaf4 antibody
    • FLJ20089 antibody
    • ING 2 antibody
    • ING 3 antibody
    • ING2 antibody
    • ING3 antibody
    • ING3_HUMAN antibody
    • Inhibitor of growth family member 3 antibody
    • Inhibitor of growth protein 3 antibody
    • MEAF4 antibody
    • p47 ING 3 antibody
    • p47 ING3 antibody
    • p47 regulator protein antibody
    • p47ING3 antibody
    • p47ING3 protein antibody
    see all

画像

  • Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

    This data was developed using ab300081, the same antibody clone in a different buffer formulation.

    ING3 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate 10 µg with ab300081 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10 µg

    Lane 2: ab300081 in Mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300081 in mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds

     

  • Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

    This data was developed using ab300081, the same antibody clone in a different buffer formulation.

    ING3 was immunoprecipitated from 0.35 mg Rat testis tissue lysate 10 µg with ab300081 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat testis tissue lysate 10 µg

    Lane 2: ab300081 in Rat testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300081 in Rat testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds

     

  • Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

    This data was developed using ab300081, the same antibody clone in a different buffer formulation.

    ING3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab300081 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

    Lane 2: ab300081 in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300081 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    Lysates should be made freshly and used in IP immediately to minimize protein degradation.

  • Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    All lanes : Anti-ING3 antibody [EPR25273-6] (ab300081) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    This data was developed using ab300081, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer. 

    Lysates should be made freshly and used in WB immediately to minimize protein degradation. 

  • Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    All lanes : Anti-ING3 antibody [EPR25273-6] (ab300081) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeting ING3, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    This data was developed using ab300081, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer. 

  • Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)
    Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300082 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300082 は論文での使用が確認できていません。

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