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Anti-Influenza A Virus Nucleoprotein 抗体 [9G8] (ab43821)

Submit a review Q&A (3)References (7)

Key features and details

  • Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
  • Suitable for: ELISA, WB, ICC/IF
  • Reacts with: Influenza A
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Influenza A Virus Nucleoprotein antibody [9G8]
    Influenza A Virus Nucleoprotein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, WB, ICC/IFmore details

  • 種交差性

    交差種: Influenza A

  • 免疫原

    Recombinant full length protein corresponding to Influenza A Virus Nucleoprotein.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    9G8

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab43821の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/2000 - 1/10000.
WB
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.

On infected cells 
特記事項
ELISA
1/2000 - 1/10000.
WB
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.

On infected cells 

ターゲット情報

  • 関連性

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

  • 細胞内局在

    Host cell nucleus

  • 参照データベース

    • 別名

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    参考文献 (7)

    ab43821 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab43821 は 7 報の論文で使用されています。

    • Dorna J  et al. Effects of Receptor Specificity and Conformational Stability of Influenza A Virus Hemagglutinin on Infection and Activation of Different Cell Types in Human PBMCs. Front Immunol 13:827760 (2022). PubMed: 35359920
    • Ganti K  et al. Rab11a mediates cell-cell spread and reassortment of influenza A virus genomes via tunneling nanotubes. PLoS Pathog 17:e1009321 (2021). PubMed: 34473799
    • Harbig A  et al. Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways. J Biol Chem 295:11388-11407 (2020). PubMed: 32303635
    • Phipps KL  et al. Collective interactions augment influenza A virus replication in a host-dependent manner. Nat Microbiol 5:1158-1169 (2020). PubMed: 32632248
    • Seo YJ  et al. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One 8:e75005 (2013). WB . PubMed: 24137500
    • Dimmock NJ  et al. Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir. Antiviral Res 96:376-85 (2012). PubMed: 23041142
    • Killingley B  et al. Virus shedding and environmental deposition of novel A (H1N1) pandemic influenza virus: interim findings. Health Technol Assess 14:237-354 (2010). PubMed: 20923613

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Thank you for your reply on this complaint.

    Please see below response from customer on your additional queries-

    Customer's Reply -

    I am surprised to know the information provided by us still not sufficient to troubleshoot.
    1. By the way the chemicon antibody is only for IFA applications. If chemicon has antibody with western blot and immunoprecipitation applications, perhaps I wouldn't be getting abcam antibody.

    2. the construct contains full NP sequence without any tag.

    3. We use H1N1, H3 N2 strains, in addition I have several seasonal isolated. all the strains been confirmed. But for this assay we used H1N1 and H3N2 strains.

    4. For your kind information in my lab every student does western blot with same detection system. We do not have any problem with detection method.

    5. I do not have purified NP protein. If I have purified protein probably I would have tried to raise antibody in the lab itself.

    Hope this information is sufficient.

    Kindly check and advise urgently as this customer is not comfortable providing additional information.

    Read More
    Answer

    Thank you for your response to my further questions. It is very much appreciated.

    When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make sure that the experiments are nicely controlled. Without these details and the exact strains of the virus, it is hard to troubleshoot and to provide sufficient technical support.

    We have not received any similar complaint about ab128193, thus not aware of any QC issues. Currently, we have the same batch in stock your customer has tried. I could offer you a new vial of ab128193 or one vial of any alternative antibodies against the same target protein (Influenza A Virus Nucleoprotein).

    We have the following antibodies which are suitable for Western blot application:

    - https://www.abcam.com/ab43821: Anti-Influenza A Virus Nucleoprotein antibody [9G8]

    - https://www.abcam.com/ab66191: Anti-Influenza A Virus Nucleoprotein antibody [B247M]

    - https://www.abcam.com/ab81126: Anti-Influenza A Virus Nucleoprotein antibody [15]

    Please could you discuss it with your customer and let me know how he/she would like to proceed.

    I look forward to hearing from you soon.

    Read More

    Question

    Dear All,
    I ordered the Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) as you stated (https://www.abcam.com/Influenza-A-Virus-Nucleoprotein-antibody-C43-ab128193.html) that it would have reacted with NP of all influenza A subtypes and the Mouse monoclonal [B247M] to influenza A virus Nucleoprotein (ab66191). I have been testing both of them in Western Blot analysis and none of them worked with the H7N3 avian influenza subtype that I am currently using for my studies. Therefore I had to use another Mab (AbCam) from a different lab. I would ask you to give me you best quotation for this Mab: “ Mouse monoclonal [9G8] to influenza A virus Nucleoprotein (ab43821)”.
    Looking forward to hear from you soon
    Best wishes,

    Read More
    Answer

    Thank you for contacting us.

    I am sorry if ab128193 did not work as you expected.

    H7N3 subtype of avian Influenza A virus has not been tested with this antibody. I would recommend you to test IF with the virus-infected cells or ELISA with inactivated purified antigen of N7H3 to examine the reactivity of C43 antibody with N7H3 subtype.

    In regards to ab43821, it should recognize H7N3 influenza; however, it was not tested on this viral strain.

    You may have received the quotation in a separate email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I would like to know the nature of the host you used to produce the recombinant nucleoprotein to raise these antibodies against. Could you please tell me if it was bacteria or some other host?

    Read More
    Answer

    ab43821 has been produced using a recombinant inlfuenza A protein produced in MDCK (Madin-Darby Canine Kidney) cells.

    Read More

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