AB228470

Anti-Indoleamine 2, 3-dioxygenase 抗体 [SP277] - N-terminal

Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. N-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

IDO, INDO, IDO1, IDO-1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) treated with IFN stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Formalin-fixed, paraffin-embedded human lung tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemical analysis of formalin fixed paraffin embedded wild type (top panels) or IDO1 knockout (lower panels) A549 cells (ab266949) labelling IDO1 with ab228470 at a concentration of 2.7µg/ml. Cells were pretreated with 25ng/ml interferon-gamma for 48hr prior to antibody incubation to induce target expression.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond^™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with NaCi buffer (pH 6.0, Epitope Retrieval Solution 1) for 20mins.

ab228470 Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal was incubated for 15mins at room temperature. Sections were counterstained with Hematoxylin. Images to the right show absence of staining with IgG isotype control antibody, rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP277

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary incubation for 10 minutes at room temperature.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

精製に関する特記事項

Purified from TCS by protein A/G.

バッファー組成

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

See full target information IDO1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 29:2939-2953 PubMed37903863

2023

An integrated gene-to-outcome multimodal database for metabolic dysfunction-associated steatotic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Kendall,Maria Jimenez-Ramos,Frances Turner,Prakash Ramachandran,Jessica Minnier,Michael D McColgan,Masood Alam,Harriet Ellis,Donald R Dunbar,Gabriele Kohnen,Prakash Konanahalli,Karin A Oien,Lucia Bandiera,Filippo Menolascina,Anna Juncker-Jensen,Douglas Alexander,Charlie Mayor,Indra Neil Guha,Jonathan A Fallowfield

View all publications

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Abcam product promise

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Anti-Indoleamine 2, 3-dioxygenase 抗体 [SP277] - N-terminal

Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (AB228470)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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組合せ製品

Assay Kits

Secondary Antibodies

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Primary Antibodies

Abcam product promise