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Anti-INCENP 抗体 (ab12183)

Reviews (6)Q&A (6)References (24)

Key features and details

  • Rabbit polyclonal to INCENP
  • Suitable for: IP, ICC, WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-INCENP antibody
    INCENP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to INCENP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, ICC, WB, ICC/IF, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Xenopus laevis
    交差が予測される動物種: Chicken

  • 免疫原

    Synthetic peptide:

    SKPRYHKRTSSAVWNSP

    (with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab12183の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
ICC (2)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

  • 配列類似性

    Belongs to the INCENP family.

  • 細胞内局在

    Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt

  • 参照データベース

    • 別名

      • binds and activates aurora B and C in vivo and in vitro antibody
      • Chromosomal passenger protein antibody
      • INCE_HUMAN antibody
      • INCENP antibody
      • Inner centromere protein antibody
      • Inner centromere protein antigens 135/155kDa antibody
      • Inner centromere protein antigens 135kD 155kD antibody
      • Inner centromere protein INCENP antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (24)

    ab12183 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab12183 は 24 報の論文で使用されています。

    • Santos-Barriopedro I  et al. Off-the-shelf proximity biotinylation using ProtA-TurboID. Nat Protoc 18:36-57 (2023). PubMed: 36224470
    • Garlapati C  et al. PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer. Cell Death Dis 14:12 (2023). PubMed: 36627281
    • Hong J  et al. Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression. J Ginseng Res 46:481-488 (2022). PubMed: 35600766
    • Pajpach F  et al. Evolution, Expression and Meiotic Behavior of Genes Involved in Chromosome Segregation of Monotremes. Genes (Basel) 12:N/A (2021). PubMed: 34573302
    • Park M  et al. A novel synthetic microtubule inhibitor exerts antiproliferative effects in multidrug resistant cancer cells and cancer stem cells. Sci Rep 11:10822 (2021). PubMed: 34031528
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-INCENP antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic Skin - keratinocytes in vivo)
    Permeabilization
    Yes - 10% Triton-X (0.05% Final)
    Specification
    Embryonic Skin - keratinocytes in vivo
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Scott Williams

    Verified customer

    投稿 Sep 30 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-INCENP antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 21 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-INCENP antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma)
    Specification
    Colon carcinoma
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    投稿 Dec 03 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-INCENP antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Methanol
    Permeabilization
    Yes - 0.1% triton X-100
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 18 2010

    Immunocytochemistry abreview for Anti-INCENP antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% in
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. William Moore

    Verified customer

    投稿 Nov 30 2005

    Immunocytochemistry abreview for Anti-INCENP antibody

     Inconclusive
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa and SK-N-SH)
    Specification
    HeLa and SK-N-SH
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    投稿 Oct 12 2005

    Question

    I would like to see the western blot used to confirm that this antibody recognises INCENP. The data sheet states that the predicted size of INCENP is 106 Kda, but does not say whether the antibody recognises a band of this size on a western blot?? I find the antibody recognises a single band of 80kDa on whole cell HeLa lysate. I see you have no independent reviews of this antibody and the technical inquiries increase my doubt that this antibody recognises INCENP. It seems unwise to continue to market this antibody as "Anti-INCENP" without better (any?) evidence that it recognises INCENP. I would appreciate any data you have on the performance of this antibody in order that I can gain something from the experiments where I have used it. It may be, for some reason, that the 80kDa band I see is INCENP, running at an unusual size. Currently I am unsure what it is! I realise that I am too late for a refund, but that is of little concern compared to the time that I and others may waste from an antibody that recognises the wrong protein! Thanks for your help,

    Read More
    Answer

    I have received confirmation from the source of ab12183 that the antibody will detect a 106 kDa band using whole HeLa extract. They cannot explain why you are detecting a 80kDa band and hypothesize that this could be a splice variant or a lysed form of INCENP. Unfortunately they were unable to provide us with an image of the blot, I apologise for the inconvenience,

    Read More

    Question

    BATCH NUMBER 63014 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM I have some doubt about whether this antibody recognises INCENP in a western blot. When used against total HeLa lysate it gives a single band of 80kDa. This band fails to disappear in lysates of siRNAi-treated HeLa, in which INCENP appears to be knocked down by western and IF with another INCENP antibody. I would be interested to hear how it has been confirmed that this antibody recognises INCENP. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? >10 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

    Read More
    Answer

    We have just received the following information from the source of ab12183 who tested the antibody in HeLa nuclear extracts: 1. Extract: 10^7 cells/slab of HeLa nuclear. 2.Wet transfer for 90 minutes at 4'C. 3. Blocking solution: 5%-10% non fat dry milk for 2-24 hours at room temperature. 4. Wash and Dilution buffer: 1% BSA in PBS-T. 5. Primary antibody: this product at 1:5,000-1:10,000 dilutions. Incubation for 120 minutes at room temperature. 6. Secondary antibody: HRP anti rabbit IgG at 1:20,000 dilution. Incubation for 60 minutes at room temperature. 7. Substrate: ECL. I hope this information helps, please do not hesitate to contact us if you need further assistance,

    Read More

    Question

    We have tried all of your suggestions and nothing is working. You suggested: - "First, I suggest running a positive control." our response - IVP is a positive control - "you did not mention what dilutions you have tried, but you may need to increase the concentration of the primary. Use a minimum dilution of 1/2000 (recommended when using a whole extract of mouse NIH-3T3 and rat PC12 cells and a chemiluminescent detection reagent)- 1/5000 (recommended when using a nuclear extract of human HeLa cells and a chemiluminescent detection reagent)." our response - we used a dilution of 1/1000 - You may also need to increase the concentration of your secondary antibody, and extend the incubation periods for both the primary and secondary. For example, incubate overnight at 4C with ab12183. our response - we icubated at 4C overnight. We would like to return the antibody and have it replaced with AB2270.

    Read More
    Answer

    Thanks for your email. I can send you ab2270 as a replacement. There's no need to return ab12183. Can you please confirm your shipping address, including phone number?

    Read More

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER Credit C DESCRIPTION OF THE PROBLEM No signal or weak signal, no band (not recognizing protein) SAMPLE IVT and IP PRIMARY ANTIBODY ab12183 (INCENP antibody) SECONDARY ANTIBODY Donkey anti-rabbit DETECTION METHOD ECL+ POSITIVE AND NEGATIVE CONTROLS USED IVT=positive control ANTIBODY STORAGE CONDITIONS 4? SAMPLE PREPARATION (MP40) / cocktail / 95? C for 10 minutes AMOUNT OF PROTEIN LOADED 1microlitre of IVT product ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE, 15% TRANSFER AND BLOCKING CONDITIONS Tris + SDS + METH / 200 MA for 1 hour and 15 minutes, 4% milk in TBST HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No ADDITIONAL NOTES verified the presence of IVT product by phosphoImager using S35

    Read More
    Answer

    Thank you for your enquiry, and I'm sorry to hear that you are experiencing difficulty with ab12183. I would like to make some suggestions in order to assist you. First, I suggest running a positive control. For this antibody nuclear/whole extract of human HeLa cells is recommended. Also, you did not mention what dilutions you have tried, but you may need to increase the concentration of the primary. Use a minimum dilution of 1/2000 (recommended when using a whole extract of mouse NIH-3T3 and rat PC12 cells and a chemiluminescent detection reagent)- 1/5000 (recommended when using a nuclear extract of human HeLa cells and a chemiluminescent detection reagent). You may also need to increase the concentration of your secondary antibody, and extend the incubation periods for both the primary and secondary. For example, incubate overnight at 4C with ab12183. If you have any additional questions, please contact us again.

    Read More

    1-10 of 12 Abreviews or Q&A

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