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リコンビナントRabMAb

Anti-IMPA1 抗体 [EPR15404(B)] (ab184165)

Reviews (2) Submit a question References (6)
Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
  • Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
  • Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
  • Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
  • Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15404(B)] to IMPA1
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-IMPA1 antibody [EPR15404(B)]
    IMPA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15404(B)] to IMPA1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HCT116 and PC3 cells; Jurkat, PC3, HCT116 aand Human testis lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184165の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
1/40 - 1/60.
ICC/IF (1)
1/100.
特記事項
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
1/40 - 1/60.
ICC/IF
1/100.

ターゲット情報

  • 機能

    Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.

  • パスウェイ

    Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.

  • 配列類似性

    Belongs to the inositol monophosphatase family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • IMP 1 antibody
    • IMP antibody
    • IMPA 1 antibody
    • IMPA antibody
    • IMPA1 antibody
    • IMPA1_HUMAN antibody
    • IMPase 1 antibody
    • IMPase antibody
    • Inositol 1(or 4) monophosphatase antibody
    • Inositol monophosphatase 1 antibody
    • Inositol monophosphatase antibody
    • Inositol(myo) 1(or 4) monophosphatase 1 antibody
    • Inositol-1(or 4)-monophosphatase 1 antibody
    • Lithium sensitive myo inositol monophosphatase A1 antibody
    • Lithium-sensitive myo-inositol monophosphatase A1 antibody
    see all

画像

  • Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    All lanes : Anti-IMPA1 antibody [EPR15404(B)] (ab184165) at 1/50000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : PC 3 cell lysate
    Lane 4 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184165 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Intracellular flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

参考文献 (6)

ab184165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184165 は 6 報の論文で使用されています。

  • Saha S  et al. IMPA1 dependent regulation of phosphatidylinositol 4,5-bisphosphate and calcium signalling by lithium. Life Sci Alliance 7:N/A (2024). PubMed: 38056909
  • Yang SY  et al. Inositol monophosphatase 1 (IMPA1) promotes triple-negative breast cancer progression through regulating mTOR pathway and EMT process. Cancer Med 12:1602-1615 (2023). PubMed: 35796646
  • Andreassi C  et al. Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep 34:108778 (2021). PubMed: 33626357
  • Pillai RA  et al. Placental Inositol Reduced in Gestational Diabetes as Glucose Alters Inositol Transporters and IMPA1 Enzyme Expression. J Clin Endocrinol Metab 106:e875-e890 (2021). PubMed: 33165596
  • Andreassi C & Riccio A A detailed protocol for RNA cleavage assay in sympathetic neurons. STAR Protoc 2:101001 (2021). PubMed: 34950884
  • Button RW  et al. Accumulation of autophagosomes confers cytotoxicity. J Biol Chem 292:13599-13614 (2017). PubMed: 28673965

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-IMPA1 antibody [EPR15404(B)]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - Triton 0.35%
Specification
Hela
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol and PFA
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 30 2015

Western blot abreview for Anti-IMPA1 antibody [EPR15404(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 30 2015

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