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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ILKAP 抗体 [EPR16145] (ab196013)

Submit a review Submit a question References (2)
Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Immunoprecipitation - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
  • Anti-ILKAP antibody [EPR16145] (ab196013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16145] to ILKAP
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ILKAP antibody [EPR16145]
    ILKAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16145] to ILKAP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK293T, HAP1, HeLa, 293, Mouse brain and Rat brain lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR16145

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab196013の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP
1/30.
特記事項
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP
1/30.

ターゲット情報

  • 機能

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.

  • 組織特異性

    Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.

  • 配列類似性

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp434J2031 antibody
    • FLJ10181 antibody
    • ILKAP antibody
    • ILKAP_HUMAN antibody
    • ILKAP2 antibody
    • ILKAP3 antibody
    • Integrin linked kinase associated phosphatase antibody
    • Integrin linked kinase associated serine/threonine phosphatase 2C antibody
    • Integrin linked kinase associated serine/threonine phosphatase antibody
    • Integrin-linked kinase-associated serine/threonine phosphatase 2C antibody
    • MGC4846 antibody
    • PP2C DELTA antibody
    • Protein phosphatase 2c delta isozyme antibody
    see all

画像

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    All lanes : Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ILKAP knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab196013 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab196013 Anti-ILKAP antibody [EPR16145] was shown to specifically react with ILKAP in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266475 (knockout cell lysate ab258000) was used. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. ab196013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ILKAP antibody [EPR16145] (ab196013)
    Immunoprecipitation - Anti-ILKAP antibody [EPR16145] (ab196013)

    Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling ILKAP using ab196013 at 1/30 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab196013 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Lane 1: Input: 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Subsequent WB detection was performed using ab196013 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ILKAP knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab196013 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab196013 was shown to recognize ILKAP when ILKAP knockout samples were used, along with additional cross-reactive bands. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. Ab196013 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/5000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Western blot - Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-ILKAP antibody [EPR16145] (ab196013)
    Anti-ILKAP antibody [EPR16145] (ab196013)

参考文献 (2)

ab196013 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab196013 は 2 報の論文で使用されています。

  • Furuke H  et al. miR‑4730 suppresses the progression of liver cancer by targeting the high mobility group A1 pathway. Int J Mol Med 49:N/A (2022). PubMed: 35485281
  • Liu L  et al. HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3ß signaling pathway. Mol Med Rep 16:9287-9294 (2017). PubMed: 29152644

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