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リコンビナントRabMAb

Anti-ILF2/NF45 抗体 [EPR10695(B)] (ab154791)

Reviews (1) Submit a question References (5)
Western blot - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Flow Cytometry (Intracellular) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
  • Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10695(B)] to ILF2/NF45
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ILF2/NF45 antibody [EPR10695(B)]
    ILF2/NF45 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10695(B)] to ILF2/NF45

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human ILF2/NF45.
    Database link: Q12905

  • ポジティブ･コントロール

    • K562, Raji, A431 and HeLa cell lysates; Human spleen tissue; Human thyroid gland carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154791の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.

  • 配列類似性

    Contains 1 DZF domain.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HGNC:6037 antibody
    • ILF 2 antibody
    • ilf2 antibody
    • ILF2_HUMAN antibody
    • Interleukin enhancer binding factor 2 antibody
    • Interleukin enhancer binding factor 2, 45kDa antibody
    • Interleukin enhancer-binding factor 2 antibody
    • MGC8391 antibody
    • NF 45 antibody
    • NF45 antibody
    • Nuclear factor of activated T cells 45 kDa antibody
    • Nuclear factor of activated T-cells 45 kDa antibody
    • PRO3063 antibody
    see all

画像

  • Western blot - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Western blot - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    All lanes : Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ILF2/NF45 with ab154791 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ILF2/NF45 with ab154791 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin embedded Human glioma tissue using ab154791 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154791 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154791 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154791 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Flow Cytometry (Intracellular) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

    Overlay histogram showing HeLa cells stained with ab154791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154791, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
    Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)

参考文献 (5)

ab154791 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154791 は 5 報の論文で使用されています。

  • Zhang J  et al. LncRNA LINC00649 promotes the growth and metastasis of triple-negative breast cancer by maintaining the stability of HIF-1α through the NF90/NF45 complex. Cell Cycle 21:1034-1047 (2022). PubMed: 35188449
  • Su Z  et al. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci 21:N/A (2020). PubMed: 32102467
  • Legrand JMD  et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun 10:2278 (2019). PubMed: 31123254
  • Li N  et al. ILF2 promotes anchorage independence through direct regulation of PTEN. Oncol Lett 18:1689-1696 (2019). PubMed: 31423236
  • Bi Y  et al. MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma. Int J Mol Med 39:900-906 (2017). WB ; Human . PubMed: 28259961

レビューと Q&A

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Western blot abreview for Anti-ILF2/NF45 antibody [EPR10695(B)]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
50 µg
Treatment
Various siRNA knockdown
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Oct 31 2013

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