Anti-ILF2/NF45 抗体 [EPR10694(B)] (ab154169)
![Western blot - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160091-anti-ilf2-antibody-epr10694b-western-blot.jpg "Western blot - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10694(B)] to ILF2/NF45
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ILF2/NF45 antibody [EPR10694(B)]
ILF2/NF45 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10694(B)] to ILF2/NF45 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ILF2/NF45.
Database link: Q12905 -
ポジティブ･コントロール
- HeLa, HepG2, Jurkat and K562 cell lysates; Human colon and kidney tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10694(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154169の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
配列類似性
Contains 1 DZF domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 3608 Human
- Entrez Gene: 67781 Mouse
- Entrez Gene: 310612 Rat
- Omim: 603181 Human
- SwissProt: Q12905 Human
- SwissProt: Q9CXY6 Mouse
- SwissProt: Q7TP98 Rat
- Unigene: 75117 Human
see all -
別名
- HGNC:6037 antibody
- ILF 2 antibody
- ilf2 antibody
see all
画像
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Western blot - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)All lanes : Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160090-anti-ilf2-antibody-epr10694b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ILF2/NF45 with ab154169 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160089-anti-ilf2-antibody-epr10694b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ILF2/NF45 with ab154169 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160088-anti-ilf2-antibody-epr10694b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin embedded Human normal stomach tissue using ab154169 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160087-anti-ilf2-antibody-epr10694b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin embedded Human normal tetis tissue using ab154169 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160086-anti-ilf2-antibody-epr10694b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154169 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-160085-anti-ilf2-antibody-epr10694b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154169 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)](https://www.abcam.co.jp/ps/products/154/ab154169/Images/ab154169-8-benefits-of-recombinant-antibodies.png)
Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154169 は 3 報の論文で使用されています。
- Watson SF et al. ILF3 contributes to the establishment of the antiviral type I interferon program. Nucleic Acids Res 48:116-129 (2020). PubMed: 31701124
- Du H et al. ILF2 Directly Binds and Stabilizes CREB to Stimulate Malignant Phenotypes of Liver Cancer Cells. Anal Cell Pathol (Amst) 2019:1575031 (2019). PubMed: 30881868
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468