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AB268075

Anti-IL3RA/CD123 抗体 [IL3RA/1531]

Anti-IL3RA/CD123 antibody [IL3RA/1531]

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Mouse Monoclonal IL3RA/CD123 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL3RA aa 1-200.

別名を表示する

CD123, IL3R, IL3RA, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)

Formalin-fixed, paraffin-embedded human gastric carcinoma tissue stained for IL3RA/CD123 using ab268075 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for IL3RA/CD123 using ab268075 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL3RA/CD123 using ab268075 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)
  • Protein Array

Unknown

Protein Array - Anti-IL3RA/CD123 antibody [IL3RA/1531] (AB268075)

Protein Array containing >19,000 full-length human proteins using ab268075. Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

IL3RA/1531

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

Protein Array, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human IL3RA aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P26951

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代替製品

Primary Antibodies

AB257307

Anti-IL3RA/CD123 antibody [IL3RA/2947R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (AB257307)

  • 0
  • 0
Primary Antibodies

AB257324

Anti-IL3RA/CD123 antibody [IL3RA/2947R] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/2947R] - BSA and Azide free (AB257324)

  • 5
  • 2
Primary Antibodies

AB21562

Anti-IL3RA/CD123 antibody [6H6]

Flow Cytometry - Anti-IL3RA/CD123 antibody [6H6] (AB21562)

  • 4
  • 7
Primary Antibodies

AB232697

PE/Cy5® Anti-IL3RA/CD123 antibody [6H6]

Flow Cytometry - PE/Cy5® Anti-IL3RA/CD123 antibody [6H6] (AB232697)

  • 0
  • 0
Primary Antibodies

AB268196

Anti-IL3RA/CD123 antibody [IL3RA/1531] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/1531] - BSA and Azide free (AB268196)

  • 2
  • 1

組合せ製品

Assay Kits

AB300317

Human IL3RA/CD123 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL3RA/CD123 ELISA Kit (AB300317)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from Bioreactor concentrate
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IL3RA also known as CD123 or interleukin-3 receptor alpha chain is a transmembrane protein with a molecular weight of approximately 70-80 kDa. This protein frequently expresses on the surface of hematopoietic cells particularly those of myeloid lineage including mast cells and basophils. Researchers use CD123 as a marker for identifying certain cell types in flow cytometry. Antibodies such as anti-CD123 are available for various applications including identifying these cells in research and clinical settings.
Biological function summary

IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.

Pathways

IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.

IL3RA expression relates closely to certain hematological conditions such as acute myeloid leukemia (AML) and systemic mastocytosis. In AML increased expression of CD123 marks leukemic cells making it a target for therapeutic intervention. The interaction of IL3RA with FLT3 another tyrosine kinase is of particular interest in these disorders as both proteins contribute to the proliferation of malignant cells through related signaling pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
See full target information IL3RA
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