Anti-IL-9 抗体 - C-terminal (ab181397)
Key features and details
- Rabbit polyclonal to IL-9 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-9 antibody - C-terminal
IL-9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-9 - C-terminal -
由来種
Rabbit -
特異性
ab181397 is predicted not to cross-react with IL7. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IL-9 (C terminal). (16 amino acid peptide; NP_000581).
Database link: P15248 -
ポジティブ･コントロール
- WB: Human spleen tissue and spleen tissue lysate; Hek 293 and HeLa lysate. IHC: Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181397の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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機能
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
配列類似性
Belongs to the IL-7/IL-9 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3578 Human
- Omim: 146931 Human
- SwissProt: P15248 Human
- Unigene: 960 Human
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別名
- Cytokine P40 antibody
- Homolog of mouse T cell and mast cell growth factor 40 antibody
- HP40 antibody
see all
画像
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Western blot - Anti-IL-9 antibody - C-terminal (ab181397)Lane 1 : Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml
Lane 2 : Anti-IL-9 antibody - C-terminal (ab181397) at 2 µg/ml
Lane 3 : Anti-IL-9 antibody - C-terminal (ab181397) at 4 µg/ml
All lanes : Human IL-9 recombinant protein, 20 ng
Secondary
All lanes : Goat anti Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 15 kDa -
Western blot - Anti-IL-9 antibody - C-terminal (ab181397)All lanes : Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml
Lane 1 : Human spleen lysate
Lane 2 : Hek 293 lysate
Lane 3 : HeLa cell spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG HRP conjugate
Predicted band size: 15 kDa -
Western blot - Anti-IL-9 antibody - Conformation-Specific (ab181397)Anti-IL-9 antibody - C-terminal (ab181397) at 1 µg/ml + Human spleen tissue lysate at 15 µg
Predicted band size: 15 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody - Conformation-Specific (ab181397)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling IL9 with ab181397 at 20 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody - Conformation-Specific (ab181397)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling IL9 with ab181397 at 5 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab181397 は 3 報の論文で使用されています。
- Kadin ME et al. Non-Malignant CD30+ Cells in Contralateral Peri-Implant Capsule of Patient With BIA-ALCL: A Premalignant Step? Aesthet Surg J N/A:N/A (2021). PubMed: 33944901
- Salazar Y et al. Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer. J Clin Invest 130:3560-3575 (2020). PubMed: 32229721
- Tong H et al. Identification of Interleukin-9 Producing Immune Cells in Endometrial Carcinoma and Establishment of a Prognostic Nomogram. Front Immunol 11:544248 (2020). PubMed: 33329510
