Anti-IL-8 抗体 (ab154390)
Key features and details
- Rabbit polyclonal to IL-8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-8 antibody
IL-8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human IL-8 aa 1-99.
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035); Human gastric carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154390の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 11 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 11 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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別名
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
画像
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Western blot - Anti-IL-8 antibody (ab154390)Anti-IL-8 antibody (ab154390) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 11 kDa
15% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody (ab154390)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling IL8 with ab154390 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab154390 は 10 報の論文で使用されています。
- Lewińska A et al. Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups. Cancers (Basel) 14:N/A (2022). PubMed: 35158873
- Huo W et al. Triclosan activates c-Jun/miR-218-1-3p/SLC35C1 signaling to regulate cell viability, migration, invasion and inflammatory response of trophoblast cells in vitro. BMC Pregnancy Childbirth 22:470 (2022). PubMed: 35668364
- Ding HM et al. miR-302c-3p and miR-520a-3p suppress the proliferation of cervical carcinoma cells by targeting CXCL8. Mol Med Rep 23:N/A (2021). PubMed: 33760117
- Wang J et al. Thioredoxin relieves lipopolysaccharide-induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis. Exp Ther Med 21:629 (2021). PubMed: 33936285
- Bloniarz D et al. The lack of functional DNMT2/TRDMT1 gene modulates cancer cell responses during drug-induced senescence. Aging (Albany NY) 13:15833-15874 (2021). PubMed: 34139673
- Lewinska A et al. AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe3O4 nanoparticles during oxidant-induced senescence in human fibroblasts. Redox Biol 28:101337 (2020). PubMed: 31622846
- Lewinska A et al. Remifentanil preconditioning protects against hypoxia-induced senescence and necroptosis in human cardiac myocytes in vitro. Aging (Albany NY) 12:13924-13938 (2020). PubMed: 32584786
- Jiang R et al. Kaempferol protects chondrogenic ATDC5 cells against inflammatory injury triggered by lipopolysaccharide through down-regulating miR-146a. Int Immunopharmacol 69:373-381 (2019). PubMed: 30776646
- Shrestha N et al. Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells. Cell Physiol Biochem 52:94-108 (2019). PubMed: 30790507
- Ba W et al. Metformin inhibits pro-inflammatory responses via targeting nuclear factor-?B in HaCaT cells. Cell Biochem Funct 37:4-10 (2019). PubMed: 30520054