Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-IL-6R 抗体 [EPR26370-132] (BSA and Azide free) (ab300582)

Submit a review Submit a question
Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
  • Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
  • Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26370-132] to IL-6R - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free)
    IL-6R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26370-132] to IL-6R - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate. Untreated mouse spleen tissue lysate, Mouse spleen tissue lysate treated with Protein Deglycosylation Mix II. IHC: Mouse thymus and spleen.

  • 特記事項

    ab300582 is the carrier-free version of ab300581.

    ab300581 does not react in IHC-Fr, immunocytochemistry, flow cytometry and immunoprecipitation. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300582の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.

  • 組織特異性

    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

  • 配列類似性

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

  • ドメイン

    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

  • 翻訳後修飾

    A short soluble form may also be released from the membrane by proteolysis.

  • 細胞内局在

    Secreted and Basolateral cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 126 antibody
      • CD126 antibody
      • CD126 antigen antibody
      • gp80 antibody
      • IL 6R 1 antibody
      • IL 6R alpha antibody
      • IL 6R antibody
      • IL-6 receptor alpha chain antibody
      • IL-6 receptor subunit alpha antibody
      • IL-6R 1 antibody
      • IL-6R subunit alpha antibody
      • IL-6R-alpha antibody
      • IL-6RA antibody
      • IL6Q antibody
      • Il6r antibody
      • IL6RA antibody
      • IL6RA_HUMAN antibody
      • IL6RQ antibody
      • Interleukin 6 receptor antibody
      • interleukin 6 receptor, alpha antibody
      • Interleukin-6 receptor subunit alpha antibody
      • Membrane glycoprotein 80 antibody
      • MGC30256 antibody
      see all

    画像

    • Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      All lanes : Anti-IL-6R antibody [EPR26370-132] (ab300581) at 1/1000 dilution

      Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 2 : EHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 70-80 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab300581, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

      Low expression: WEHI-231(PMID: 2112585)
      The identity of the lower MW band at approximately 40 kDa (in lane 1) is unknown.

    • Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      Western blot - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      All lanes : Anti-IL-6R antibody [EPR26370-132] (ab300581) at 1/1000 dilution

      Lane 1 : Untreated mouse spleen tissue lysate
      Lane 2 : Mouse spleen tissue lysate treated with Protein Deglycosylation Mix II

      Lysates/proteins at 15 µg per lane.

      Secondary
      Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
      Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051))

      Observed band size: 55, 70-80 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab300581, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

      Target is a glycoprotein of approximately 75 kDa and detected as a 55kDa band after treated with Protein Deglycosylation MIX II. 

      Exposure time: 180 seconds. 

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)

      This data was developed using ab300581, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse thymus is observed. The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)

      This data was developed using ab300581, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen is observed (PMID: 23378927). The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead od primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used. 

    • Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)
      Rabbit monoclonal [EPR26370-132] to Interleukin-6 receptor subunit alpha - BSA and Azide free (AB300582)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300582 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300582 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300582.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com